BMRB: 51139

ASCC2 CUE domain interaction with K63-linked diubiquitin

Authors

Lombardi, P.M., Anoh, R., Burke, K.A., Schmelyun, D.P., Majumdar, A., Wolberger, C.

Assembly

ASCC2 CUE:K63-linked diubiquitin

Entity

1. ASCC2 CUE:K63-linked diubiquitin, entity 1 (polymer, Thiol state: all free), 55 monomers, 6331.075 Da Detail

ELDSLISQVK DLLPDLGEGF ILACLEYYHY DPEQVINNIL EERLAPTLSQ LDRNL

2. ASCC2 CUE:K63-linked diubiquitin, entity 2 (polymer, Thiol state: not present), 153 monomers, 17282.56 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGRQL EDGRTLSDYN IQRESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR GGD

Total weight

23613.637 Da

Max. entity weight

17282.56 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 26.0 %, Completeness: 7.8 %, Completeness (bb): 17.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	7.8 % (119 of 1531)	5.1 % (66 of 1298)	22.7 % (53 of 233)
Backbone	17.6 % (109 of 620)	13.3 % (56 of 421)	26.6 % (53 of 199)
Sidechain	0.9 % (8 of 911)	0.9 % (8 of 877)	0.0 % (0 of 34)
Aromatic	7.8 % (4 of 51)	7.8 % (4 of 51)
Methyl	0.0 % (0 of 137)	0.0 % (0 of 137)

1. entity 1

ELDSLISQVK DLLPDLGEGF ILACLEYYHY DPEQVINNIL EERLAPTLSQ LDRNL

2. entity 2

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGRQL EDGRTLSDYN IQRESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR GGD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 313 K, pH 7.0, Details in a buffer consisting of 20 mM Tris pH 7.0, 100 mM NaCl, and 200 mM TCEP

#	Name	Isotope labeling	Type	Concentration
1	ASCC2 CUE domain	[U-15N]		20 uM
2	K63-linked diubiquitin	natural abundance		40 uM
3	Tris	natural abundance		20 mM
4	NaCl	natural abundance		100 mM
5	TCEP	natural abundance		200 mM

Sample #2

Solvent system 90% H2O/10% D2O, Temperature 313 K, pH 7.0, Details in a buffer consisting of 20 mM Tris pH 7.0, 100 mM NaCl, and 200 mM TCEP

#	Name	Isotope labeling	Type	Concentration
6	ASCC2 CUE domain	[U-15N]		20 uM
7	K63-linked diubiquitin	natural abundance		20 uM
8	Tris	natural abundance		20 mM
9	NaCl	natural abundance		100 mM
10	TCEP	natural abundance		200 mM

Sample #3

Solvent system 90% H2O/10% D2O, Temperature 313 K, pH 7.0, Details in a buffer consisting of 20 mM Tris pH 7.0, 100 mM NaCl, and 200 mM TCEP

#	Name	Isotope labeling	Type	Concentration
11	ASCC2 CUE domain	[U-15N]		20 uM
12	K63-linked diubiquitin	natural abundance		6 uM
13	Tris	natural abundance		20 mM
14	NaCl	natural abundance		100 mM
15	TCEP	natural abundance		200 mM

Sample #4

Solvent system 90% H2O/10% D2O, Temperature 313 K, pH 7.0, Details in a buffer consisting of 20 mM Tris pH 7.0, 100 mM NaCl, and 200 mM TCEP

#	Name	Isotope labeling	Type	Concentration
16	ASCC2 CUE domain	[U-15N]		20 uM
17	Tris	natural abundance		20 mM
18	NaCl	natural abundance		100 mM
19	TCEP	natural abundance		200 mM

Hide sample codition

Release date

2021-10-13

Citation

The ASCC2 CUE domain in the ALKBH3-ASCC DNA repair complex recognizes adjacent ubiquitins in K63-linked polyubiquitin
Lombardi, P.M., Haile, S., Rusanov, T., Rodell, R., Anoh, R., Baer, J.G., Burke, K.A., Gray, L.N., Hacker, A.R., Kebreau, K.R., Ngandu, C.K., Orland, H.A., Osei-Asante, E., Schmelyun, D.P., Shorb, D.E., Syed, S.H., Veilleux, J.M., Majumdar, A., Mosammaparast, N., Wolberger, C.
J. Biol. Chem. (2022), 298, 101545-101545, PubMed 34971705 , DOI 10.1016/j.jbc.2021.101545 , Abstract

Related entities 1. ASCC2 CUE:K63-linked diubiquitin, entity 1, : 1 : 2 : 12 entities Detail

Related entities 2. ASCC2 CUE:K63-linked diubiquitin, entity 2, : 221 entities Detail

Interaction partners 1. ASCC2 CUE:K63-linked diubiquitin, entity 1, : 49 interactors Detail

Experiments performed 1 experiments Detail