BMRB: 5117

Structural and Dynamic Differences of Rhodostomin, an RGD-containing Disintegrin, and its D51E mutant

Authors

Chuang, W.

Assembly

Rhodostomin

Entity

1. Rhodostomin (polymer, Thiol state: all disulfide bound), 76 monomers, 8429.390 Da Detail

EFHHHHHHGK ECDCSSPENP CCDAATCKLR PGAQCGEGLC CEQCKFSRAG KICRIPRGDM PDDRCTGQSA DCPRYH

Formula weight

8429.39 Da

Entity Connection

disulfide 6 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS12:SG	1:CYS27:SG
2	disulfide	sing	1:CYS14:SG	1:CYS22:SG
3	disulfide	sing	1:CYS21:SG	1:CYS44:SG
4	disulfide	sing	1:CYS35:SG	1:CYS41:SG
5	disulfide	sing	1:CYS40:SG	1:CYS65:SG
6	disulfide	sing	1:CYS53:SG	1:CYS72:SG

Source organism

Calloselasma rhodostoma

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.5 %, Completeness: 81.6 %, Completeness (bb): 87.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	81.6 % (412 of 505)	80.3 % (346 of 431)	89.2 % (66 of 74)
Backbone	87.9 % (196 of 223)	87.6 % (134 of 153)	88.6 % (62 of 70)
Sidechain	76.6 % (216 of 282)	76.3 % (212 of 278)	100.0 % (4 of 4)
Aromatic	28.6 % (8 of 28)	28.6 % (8 of 28)
Methyl	100.0 % (15 of 15)	100.0 % (15 of 15)

1. Rhodostomin

EFHHHHHHGK ECDCSSPENP CCDAATCKLR PGAQCGEGLC CEQCKFSRAG KICRIPRGDM PDDRCTGQSA DCPRYH

Show sample condition

Sample #1

Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	Rhodostomin			3.0 mM

Sample #2

Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
2	Rhodostomin	[U-98% 15N]		2.5 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2PJF, Strand ID: A Detail

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Release date

2003-04-08

Citation

Expression in Pichia pastoris and characterization by circular dichroism and NMR of rhodostomin
Guo, R., Chou, L., Chen, Y., Chen, C., Pari, K., Jen, C., Lo, S., Huang, S., Lee, C., Chang, T., Chuang, W.
Proteins (2001), 43, 499-508, PubMed 11340665 , DOI 10.1002/prot.1061 , Abstract

Related entities 1. Rhodostomin, : 1 : 14 : 205 entities Detail

Experiments performed 7 experiments Detail