BMRB: 51286

CLAMP-interacting disordered region of male-specific lethal protein 2 (Msl2)

Authors

Mariasina, S.S.S., Bonchuk, A.A.N., Polshakov, V.V.I.

Assembly

Msl2

Entity

1. Msl2 (polymer, Thiol state: all free), 42 monomers, 4625.111 Da Detail

SGSISLVPLN NLQQSQHPLV LVQNEKGEYQ GFNIFQGSKP LD

Formula weight

4625.111 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 55.7 %, Completeness (bb): 93.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	55.7 % (279 of 501)	48.5 % (128 of 264)	60.1 % (113 of 188)	77.6 % (38 of 49)
Backbone	93.1 % (229 of 246)	91.8 % (78 of 85)	92.6 % (113 of 122)	97.4 % (38 of 39)
Sidechain	28.7 % (84 of 293)	27.9 % (50 of 179)	32.7 % (34 of 104)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 32)	0.0 % (0 of 16)	0.0 % (0 of 16)
Methyl	11.4 % (5 of 44)	22.7 % (5 of 22)	0.0 % (0 of 22)

1. entity 1

SGSISLVPLN NLQQSQHPLV LVQNEKGEYQ GFNIFQGSKP LD

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Msl2	[U-98% 13C; U-98% 15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	D2O	[U-2H]		5 %

Hide sample codition

Release date

2022-01-20

Citation

Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila
Tihonova, E., Mariasina, S., Efimov, S., Polshakov, V., Maksimenko, O., Georgiev, P., Bonchuk, A.
Nucleic Acids Res. (2022), 50, 6521-6531, PubMed 35648444 , DOI 10.1093/nar/gkac455 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Msl2, : 2 : 1 entities Detail

Interaction partners 1. Msl2, : 1 interactors Detail

Experiments performed 5 experiments Detail