BMRBj - BMREntryMaster

Found 1 Document (0.201 seconds)

BMRB: 51350

NPSL2

Authors

Liu, Y., Keane, S.C.

Assembly

NPSL2

Entity

1. NPSL2 (polymer, Thiol state: not present), 43 monomers, 13869.28 Da Detail

GGGUGUAGAA AAGUAAGGGA AACUCAAACC CCUUUCUACA CCC

Formula weight

13869.28 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 39.9 %, Completeness (bb): 27.3 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C
All	39.9 % (266 of 666)	60.7 % (227 of 374)	13.4 % (39 of 292)
Suger, PO4	27.3 % (129 of 473)	50.0 % (129 of 258)	0.0 % (0 of 215)
Nucleobase	71.0 % (137 of 193)	84.5 % (98 of 116)	50.6 % (39 of 77)
Aromatic	75.4 % (129 of 171)	95.7 % (90 of 94)	50.6 % (39 of 77)

1. entity 1

GGGUGUAGAA AAGUAAGGGA AACUCAAACC CCUUUCUACA CCC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	NPSL2	natural abundance		500 uM
2	potassium phosphate	natural abundance		50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	NPSL2	natural abundance		500 uM
4	potassium phosphate	natural abundance		50 mM

Release date

2022-03-06

Citation

Solution structure of NPSL2, a regulatory element in the oncomiR-1 RNA
Liu, Y., Munsayac, A., Hall, I., Keane, S.C.
J. Mol. Biol.

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr51350_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr51350_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	270		100.0 (chain: 1, length: 43)
1	Chemical shift references	chem_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 43, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 231
  - ¹³C chemical shifts: 39
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
3	G	H21	8.3893
5	G	H21	8.2794
40	A	H61	7.9855
40	A	H62	6.3089

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Found 1 Document (0.201 seconds)

BMRB: 51350

Sample #1

Sample #2

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail