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BMRB: 51389

1H and 13C chemical shifts for a peptide encompassing residues 2-19 of the human formin INF2 in aqueous solution

Authors

Jimenez, M.

Assembly

INF2(2-19)

Entity

1. INF2(2-19) (polymer, Thiol state: not present), 19 monomers, 1997.322 Da Detail

SVKEGAQRKW AALKEKLGX

Formula weight

1997.322 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 89.5 %, Completeness: 82.4 %, Completeness (bb): 72.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	82.4 % (164 of 199)	90.6 % (106 of 117)	70.7 % (58 of 82)
Backbone	72.2 % (65 of 90)	84.2 % (32 of 38)	63.5 % (33 of 52)
Sidechain	91.2 % (114 of 125)	93.7 % (74 of 79)	87.0 % (40 of 46)
Aromatic	100.0 % (11 of 11)	100.0 % (6 of 6)	100.0 % (5 of 5)
Methyl	77.8 % (14 of 18)	77.8 % (7 of 9)	77.8 % (7 of 9)

1. entity 1

SVKEGAQRKW AALKEKLGX

Show sample condition

Sample

Solvent system water, Pressure 1 atm, Temperature 278 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	INF2(2-19)	natural abundance	1 mM
2	D2O	[U-2H]	10 % v/v
3	H2O	natural abundance	90 % v/v
4	DSS	natural abundance	0.1 mM

Chem. Shift Complete2

Sequence coverage: 89.5 %, Completeness: 81.9 %, Completeness (bb): 72.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	81.9 % (326 of 398)	89.7 % (210 of 234)	70.7 % (116 of 164)
Backbone	72.8 % (131 of 180)	86.8 % (66 of 76)	62.5 % (65 of 104)
Sidechain	90.0 % (225 of 250)	91.1 % (144 of 158)	88.0 % (81 of 92)
Aromatic	100.0 % (22 of 22)	100.0 % (12 of 12)	100.0 % (10 of 10)
Methyl	88.9 % (32 of 36)	88.9 % (16 of 18)	88.9 % (16 of 18)

1. entity 1

SVKEGAQRKW AALKEKLGX

Show sample condition

Sample

Solvent system water, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	INF2(2-19)	natural abundance	1 mM
2	D2O	[U-2H]	10 % v/v
3	H2O	natural abundance	90 % v/v
4	DSS	natural abundance	0.1 mM

Release date

2022-04-05

Citation

Structure and function of the N-terminal extension of the formin INF2
Labat-de-Hoz, L., Comas, L., Rubio-Ramos, A., Casares-Arias, J., Fernandez-Martin, L., Pantoja-Uceda, D., Martin, M., Kremer, L., Jimenez, M., Correas, I., Alonso, M.A.
Cell. Mol. Life Sci. (2022), 79, 571-571, PubMed 36306014 , DOI 10.1007/s00018-022-04581-y , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 51422 released on 2022-04-27
    Title 1H, 13C and 15N backbone chemical shifts for human calmodulin in aqueous solution
  BMRB: 51407 released on 2022-04-21
    Title 1H and 13C chemical shifts for a peptide encompassing residues 2-36 of the human formin INF2 in aqueous solution
  BMRB: 51408 released on 2022-04-21
    Title 1H and 13C chemical shifts for a peptide encompassing residues 2-36 of the human formin INF2 in 30% TFE
  BMRB: 51388 released on 2022-04-05
    Title 1H and 13C chemical shifts for a peptide encompassing residues 2-19 of the human formin INF2 in 30% TFE

Related entities 1. INF2(2-19), : 5 : 2 entities Detail

Interaction partners 1. INF2(2-19), : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 1D 1H