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BMRB: 51521

8BDV

Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus

Authors

Garaeva, N., Bikmullin, A., Fatkhullin, B., Validov, S., Kieffer, B., Yusupov, M., Usachev, K.

Assembly

RimP

Entity

1. RimP (polymer, Thiol state: unknown), 156 monomers, 17698.15 Da Detail

MASKITEQVE VIVKPIMEDL NFELVDVEYV KEGRDHFLRI SIDKEGGVDL NDCTLASEKI SEAMDANDPI PEMYYLDVAS PGAERPIKKE QDFQNAITKP VFVSLYVPIE GEKEWLGILQ EVNNETIVVQ VKIKARTKDI EIPRDKIAKA RHAVMI

Formula weight

17698.15 Da

Source organism

Escherichia coli

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 66.5 %, Completeness (bb): 90.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.5 % (1236 of 1859)	57.7 % (559 of 968)	73.8 % (535 of 725)	85.5 % (142 of 166)
Backbone	90.8 % (835 of 920)	90.3 % (280 of 310)	90.0 % (416 of 462)	93.9 % (139 of 148)
Sidechain	48.7 % (530 of 1089)	41.9 % (276 of 658)	61.0 % (252 of 413)	11.1 % (2 of 18)
Aromatic	0.0 % (0 of 92)	0.0 % (0 of 46)	0.0 % (0 of 45)	0.0 % (0 of 1)
Methyl	55.5 % (111 of 200)	43.0 % (43 of 100)	68.0 % (68 of 100)

1. entity 1

MASKITEQVE VIVKPIMEDL NFELVDVEYV KEGRDHFLRI SIDKEGGVDL NDCTLASEKI SEAMDANDPI PEMYYLDVAS PGAERPIKKE QDFQNAITKP VFVSLYVPIE GEKEWLGILQ EVNNETIVVQ VKIKARTKDI EIPRDKIAKA RHAVMI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

Release date

2022-07-19

Citation

Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus
Garaeva, N., Bikmullin, A., Fatkhullin, B., Validov, S., Kieffer, B., Yusupov, M., Usachev, K.
Biomol. NMR Assign. (2022), 16, 373-377, PubMed 36070063 , DOI 10.1007/s12104-022-10106-2 , Abstract

Related entities 1. RimP, : 1 : 1 : 2 : 110 entities Detail

Experiments performed 14 experiments Detail

1 1D 1H

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

2 1D 13C

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

3 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

4 2D 1H-13C HMQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

5 3D CBCACONH

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

6 3D CBCANH

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

7 3D HNCACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

8 3D HN(CO)CACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

9 3D HNCO

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

10 3D HNCA

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

11 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

12 3D H(CCO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

13 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

14 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-100% 13C; U-100% 15N]		0.7 mM
2	TrisHCl buffer	natural abundance		10 mM
3	NaCl	natural abundance		250 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr51521_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr51521_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1209		92.3 (chain: 1, length: 156)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 156, Sequence coverage: 92.3%
- Total number of assignments
  - ¹³C chemical shifts: 502
  - ¹H chemical shifts: 570
  - ¹⁵N chemical shifts: 137
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1