BMRB: 5199

Assignment of the 1H and 15N resonances of the elicitor protein NIP1

Authors

van't Slot, K.A.E., van den Burg, H.A., Kloks, C.P.A.M., Hilbers, C.W., Knogge, W., Papavoine, C.H.M.

Assembly

necrosis inducing protein

Entity

1. necrosis inducing protein (polymer, Thiol state: not reported), 60 monomers, 6443.297 Da Detail

DRCRYTLCCD GALKAVSACL HESESCLVPG DCCRGKSRLT LCSYGEGGNG FQCPTGYRQC

Formula weight

6443.297 Da

Entity Connection

disulfide 5 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS19:SG
2	disulfide	sing	1:CYS8:SG	1:CYS26:SG
3	disulfide	sing	1:CYS9:SG	1:CYS42:SG
4	disulfide	sing	1:CYS32:SG	1:CYS53:SG
5	disulfide	sing	1:CYS33:SG	1:CYS60:SG

Source organism

Rhynchosporium secalis

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.0 %, Completeness (bb): 98.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	99.0 % (384 of 388)	99.1 % (324 of 327)	98.4 % (60 of 61)
Backbone	98.9 % (182 of 184)	99.2 % (125 of 126)	98.3 % (57 of 58)
Sidechain	99.0 % (202 of 204)	99.0 % (199 of 201)	100.0 % (3 of 3)
Aromatic	89.5 % (17 of 19)	89.5 % (17 of 19)
Methyl	100.0 % (22 of 22)	100.0 % (22 of 22)

1. Necrosis Inducing Protein 1

DRCRYTLCCD GALKAVSACL HESESCLVPG DCCRGKSRLT LCSYGEGGNG FQCPTGYRQC

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Necrosis Inducing Protein 1	[U-15N]		2.0 mM

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Protein Blocks Logo

Calculated from 25 models in PDB: 1KG1, Strand ID: A Detail

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Coupling constant

35 J values in 1 lists
Temperature 298 (±1) K, pH 6.0 (±0.2) Detail

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Temperature 298 (±1) K, pH 6.0 (±0.2)

List #1 nip1_J-couplings

Release date

2003-06-08

Citation

Solution structure of the fungal plant disease resistance-triggering protein NIP1 shows a novel b sheet fold
van't Slot, K.A.E., van den Burg, H.A., Kloks, C.P.A.M., Hilbers, C.W., Knogge, W., Papavoine, C.H.M.

Related entities 1. necrosis inducing protein, : 1 entities Detail

Experiments performed 10 experiments Detail