BMRB: 52052

Residual dipolar couplings measured on HIV-1 TAR ES1 mutant C30U using Pf1 phage alignment media for validating FARFAR-NMR ensemble

Authors

Roy, R., Geng, A., Shi, H., Merriman, D.K., Dethoff, E.A., Salmon, L., Al-Hashimi, H.M.

Assembly

Monomeric RNA

Entity

1. Monomeric RNA (polymer, Thiol state: not present), 20 monomers, 6532.866 Da Detail

GGCGAGCUUG GGAGCUCGCC

Formula weight

6532.866 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

RDCs

RDC

16 RDC values in 1 lists
Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 298 K, pH 6.4 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.4

Experiment name 2D 13C-1H TROSY HSQC, 2D 13C-1H S3CT HSQC

List #1 RDCs_1, RDC code DCH, Field strength (¹H) 800 MHz, Details Aligned using 25 mg/ml Pf1 phage media

Release date

2023-07-23

Citation

Kinetic Resolution of the Atomic 3D Structures Formed by Ground and Excited Conformational States in an RNA Dynamic Ensemble
Roy, R., Geng, A., Shi, H., Merriman, D.K., Dethoff, E.A., Salmon, L., Al-Hashimi, H.M.
J. Am. Chem. Soc. (2023), 145, 22964-22978, PubMed 37831584 , DOI 10.1021/jacs.3c04614 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 4 experiments Detail