BMRB: 5218

Backbone 1H, 13C, 15N resonance assignments of the N-terminal 24 kDa fragment of the gyrase B subunit from E. coli

Authors

Bellanda, M., Peggion, E., Otting, G., Weigelt, J., Perdona, E., Domenici, E., Marchioro, C., Mammi, S.

Assembly

24 kDa N-terminal fragment of Gyrase B

Entity

1. 24 kDa N-terminal fragment of Gyrase B (polymer, Thiol state: all free), 220 monomers, 24156.87 Da Detail

MSNSYDSSSI KVLKGLDAVR KRPGMYIGDT DDGTGLHHMV FEVVDNAIDE ALAGHCKEII VTIHADNSVS VQDDGRGIPT GIHPEEGVSA AEVIMTVLHA GGKFDDNSYK VSGGLHGVGV SVVNALSQKL ELVIQREGKI HRQIYEHGVP QAPLAVTGET EKTGTMVRFW PSLETFTNVT EFEYEILAKR LRELSFLNSG VSIRLRDKRD GKEDHFHYEG

Formula weight

24156.87 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 49.1 %, Completeness (bb): 87.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	49.1 % (1215 of 2473)	34.9 % (445 of 1274)	58.7 % (571 of 972)	87.7 % (199 of 227)
Backbone	87.8 % (1149 of 1308)	85.1 % (389 of 457)	88.2 % (562 of 637)	92.5 % (198 of 214)
Sidechain	16.5 % (225 of 1362)	7.0 % (57 of 817)	31.4 % (167 of 532)	7.7 % (1 of 13)
Aromatic	0.6 % (1 of 174)	1.1 % (1 of 87)	0.0 % (0 of 86)	0.0 % (0 of 1)
Methyl	9.4 % (24 of 256)	7.0 % (9 of 128)	11.7 % (15 of 128)

1. 24 kDa N-terminal fragment of DNA Gyrase

MSNSYDSSSI KVLKGLDAVR KRPGMYIGDT DDGTGLHHMV FEVVDNAIDE ALAGHCKEII VTIHADNSVS VQDDGRGIPT GIHPEEGVSA AEVIMTVLHA GGKFDDNSYK VSGGLHGVGV SVVNALSQKL ELVIQREGKI HRQIYEHGVP QAPLAVTGET EKTGTMVRFW PSLETFTNVT EFEYEILAKR LRELSFLNSG VSIRLRDKRD GKEDHFHYEG

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	24 kDa N-terminal fragment of DNA Gyrase	[U-13C; U-15N]		1.0 mM
2	sodium phosphate			20 mM
3	KCl			40 mM
4	NaCl			40 mM
5	EDTA			1 mM
6	DTT			2 mM
7	sodium azide			0.02 % w/v

Sample #2

Temperature 298 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	24 kDa N-terminal fragment of DNA Gyrase	[U-75% 2H; U-13C; U-15N]		1.2 mM
9	sodium phosphate			10 mM
10	KCl			20 mM
11	EDTA			1 mM
12	DTT			2 mM
13	sodium azide			0.02 % w/v

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LACS Plot; CA

Referencing offset: 0.36 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.36 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.37 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Release date

2002-08-21

Citation 1

Backbone 1H, 13C and 15N resonance assignment of the N-terminal 24 kDa fragment of the gyrase B subunit from E. coli
Bellanda, M., Peggion, E., Otting, G., Weigelt, J., Perdona, E., Domenici, E., Marchioro, C., Mammi, S.
J. Biomol. NMR (2002), 22, 369-370, PubMed 12018484 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI 10.1007/bf00211777 , Abstract

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Related entities 1. 24 kDa N-terminal fragment of Gyrase B, : 1 : 24 : 40 : 153 entities Detail

Interaction partners 1. 24 kDa N-terminal fragment of Gyrase B, : 1 interactors Detail

Chemical shift validation 3 contents Detail