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BMRB: 52226

8RHS

NMR chemical shifts of the human Roquin-1 ZnF domain

Authors

Tants, J., Schlundt, A.

Assembly

Znf

Entity

1. Znf, entity 1 (polymer, Thiol state: all free), 48 monomers, 5568.467 Da Detail

GAMAHSKYKT YMCRDMKQRG GCPRGASCTF AHSQEELEKF RKMNKRLV

2. Znf, entity ZN (non-polymer), 65.409 Da

Total weight

5633.876 Da

Max. entity weight

5568.467 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 324, ¹³C: 208, ¹⁵N: 43 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	GLY	HA2	H	4.105	0.015	2
2	1	1	GLY	HA3	H	3.709	0.016	2
3	1	1	GLY	CA	C	45.275	0.045	1
4	1	2	ALA	HA	H	4.205	0.029	1
5	1	2	ALA	HB1	H	1.282	0.025	1
6	1	2	ALA	HB2	H	1.282	0.025	1
7	1	2	ALA	HB3	H	1.282	0.025	1
8	1	2	ALA	C	C	177.631	0.000	1
9	1	2	ALA	CA	C	52.516	0.074	1
10	1	2	ALA	CB	C	19.246	0.086	1
11	1	3	MET	HE1	H	1.850	0.027	1
12	1	3	MET	HE2	H	1.850	0.027	1
13	1	3	MET	HE3	H	1.850	0.027	1
14	1	3	MET	CE	C	16.778	0.038	1
15	1	4	ALA	HA	H	4.185	0.006	1
16	1	4	ALA	HB1	H	1.269	0.022	1
17	1	4	ALA	HB2	H	1.269	0.022	1
18	1	4	ALA	HB3	H	1.269	0.022	1
19	1	4	ALA	C	C	179.083	0.000	1
20	1	4	ALA	CA	C	52.500	0.069	1
21	1	4	ALA	CB	C	19.078	0.065	1
22	1	5	HIS	HA	H	4.714	0.000	1
23	1	5	HIS	HD2	H	6.910	0.000	1
24	1	5	HIS	HE1	H	7.772	0.116	1
25	1	5	HIS	HE2	H	7.339	0.000	1
26	1	5	HIS	C	C	178.292	0.000	1
27	1	5	HIS	CD2	C	120.222	0.000	1
28	1	5	HIS	CE1	C	138.437	0.087	1
29	1	6	SER	HA	H	4.308	0.001	1
30	1	6	SER	HB2	H	3.797	0.030	2
31	1	6	SER	HB3	H	3.963	0.006	2
32	1	6	SER	C	C	177.660	0.000	1
33	1	6	SER	CA	C	58.489	0.174	1
34	1	6	SER	CB	C	63.648	0.107	1
35	1	7	LYS	HA	H	4.203	0.022	1
36	1	7	LYS	HB2	H	1.722	0.013	2
37	1	7	LYS	HB3	H	1.721	0.015	2
38	1	7	LYS	HG2	H	1.348	0.021	1
39	1	7	LYS	HG3	H	1.348	0.021	1
40	1	7	LYS	HD2	H	1.581	0.029	2
41	1	7	LYS	HD3	H	1.833	0.021	2
42	1	7	LYS	HE2	H	2.883	0.013	2
43	1	7	LYS	HE3	H	2.897	0.000	2
44	1	7	LYS	C	C	176.606	0.000	1
45	1	7	LYS	CA	C	56.359	0.077	1
46	1	7	LYS	CB	C	32.538	0.134	1
47	1	7	LYS	CG	C	24.837	0.141	1
48	1	7	LYS	CD	C	28.815	0.192	1
49	1	7	LYS	CE	C	42.112	0.010	1
50	1	8	TYR	H	H	7.912	0.004	1
51	1	8	TYR	HA	H	4.168	0.040	1
52	1	8	TYR	HB2	H	2.657	0.003	2
53	1	8	TYR	HB3	H	2.646	0.010	2
54	1	8	TYR	HD1	H	6.720	0.005	1
55	1	8	TYR	HD2	H	6.713	0.003	1
56	1	8	TYR	HE1	H	6.445	0.009	1
57	1	8	TYR	HE2	H	6.445	0.009	1
58	1	8	TYR	C	C	174.105	0.000	1
59	1	8	TYR	CA	C	59.223	0.117	1
60	1	8	TYR	CB	C	38.345	0.073	1
61	1	8	TYR	CD1	C	132.874	0.084	1
62	1	8	TYR	CD2	C	132.874	0.084	1
63	1	8	TYR	CE1	C	117.562	0.153	1
64	1	8	TYR	CE2	C	117.562	0.153	1
65	1	8	TYR	N	N	122.820	0.033	1
66	1	9	LYS	H	H	7.943	0.021	1
67	1	9	LYS	HA	H	3.370	0.007	1
68	1	9	LYS	HB2	H	1.220	0.009	2
69	1	9	LYS	HB3	H	2.337	0.023	2
70	1	9	LYS	HG2	H	1.450	0.027	2
71	1	9	LYS	HG3	H	1.320	0.039	2
72	1	9	LYS	HD2	H	1.591	0.026	1
73	1	9	LYS	HD3	H	1.591	0.026	1
74	1	9	LYS	HE2	H	2.926	0.034	1
75	1	9	LYS	HE3	H	2.926	0.034	1
76	1	9	LYS	HZ1	H	7.346	0.000	1
77	1	9	LYS	HZ2	H	7.346	0.000	1
78	1	9	LYS	HZ3	H	7.346	0.000	1
79	1	9	LYS	C	C	173.229	0.000	1
80	1	9	LYS	CA	C	58.041	0.118	1
81	1	9	LYS	CB	C	31.394	0.113	1
82	1	9	LYS	CG	C	25.220	0.097	1
83	1	9	LYS	CD	C	29.702	0.193	1
84	1	9	LYS	CE	C	42.121	0.000	1
85	1	9	LYS	N	N	120.524	0.176	1
86	1	10	THR	H	H	7.413	0.016	1
87	1	10	THR	HA	H	4.206	0.050	1
88	1	10	THR	HB	H	4.041	0.035	1
89	1	10	THR	HG21	H	0.908	0.020	1
90	1	10	THR	HG22	H	0.908	0.020	1
91	1	10	THR	HG23	H	0.908	0.020	1
92	1	10	THR	C	C	173.479	0.000	1
93	1	10	THR	CA	C	61.784	0.184	1
94	1	10	THR	CB	C	69.076	0.073	1
95	1	10	THR	CG2	C	21.781	0.045	1
96	1	10	THR	N	N	103.534	0.051	1
97	1	11	TYR	H	H	7.803	0.024	1
98	1	11	TYR	HA	H	4.687	0.019	1
99	1	11	TYR	HB2	H	2.887	0.025	2
100	1	11	TYR	HB3	H	3.111	0.028	2
101	1	11	TYR	HD1	H	7.155	0.027	1
102	1	11	TYR	HD2	H	7.155	0.027	1
103	1	11	TYR	HE1	H	6.741	0.009	1
104	1	11	TYR	HE2	H	6.719	0.000	1
105	1	11	TYR	C	C	175.188	0.000	1
106	1	11	TYR	CA	C	56.680	0.133	1
107	1	11	TYR	CB	C	41.563	0.192	1
108	1	11	TYR	CD1	C	133.455	0.035	1
109	1	11	TYR	CD2	C	133.455	0.035	1
110	1	11	TYR	CE1	C	117.985	0.000	1
111	1	11	TYR	CE2	C	117.985	0.000	1
112	1	11	TYR	N	N	119.621	0.127	1
113	1	12	MET	H	H	8.883	0.018	1
114	1	12	MET	HA	H	4.475	0.024	1
115	1	12	MET	HB2	H	1.851	0.016	2
116	1	12	MET	HB3	H	1.846	0.000	2
117	1	12	MET	HG2	H	2.514	0.043	2
118	1	12	MET	HG3	H	2.529	0.043	2
119	1	12	MET	HE1	H	1.741	0.028	1
120	1	12	MET	HE2	H	1.741	0.028	1
121	1	12	MET	HE3	H	1.741	0.028	1
122	1	12	MET	C	C	175.888	0.000	1
123	1	12	MET	CA	C	54.304	0.095	1
124	1	12	MET	CB	C	31.696	0.163	1
125	1	12	MET	CG	C	31.226	0.655	1
126	1	12	MET	CE	C	15.998	0.041	1
127	1	12	MET	N	N	122.685	0.070	1
128	1	13	CYS	H	H	8.923	0.011	1
129	1	13	CYS	HA	H	3.516	0.034	1
130	1	13	CYS	HB2	H	2.651	0.021	2
131	1	13	CYS	HB3	H	2.964	0.031	2
132	1	13	CYS	C	C	176.332	0.000	1
133	1	13	CYS	CA	C	61.133	0.112	1
134	1	13	CYS	CB	C	31.328	0.139	1
135	1	13	CYS	N	N	126.460	0.092	1
136	1	14	ARG	H	H	8.493	0.009	1
137	1	14	ARG	HA	H	3.985	0.037	1
138	1	14	ARG	HB2	H	1.585	0.027	2
139	1	14	ARG	HB3	H	1.581	0.000	2
140	1	14	ARG	HG2	H	1.564	0.008	1
141	1	14	ARG	HG3	H	1.564	0.008	1
142	1	14	ARG	HD2	H	3.126	0.017	1
143	1	14	ARG	HD3	H	3.126	0.017	1
144	1	14	ARG	C	C	177.475	0.000	1
145	1	14	ARG	CA	C	58.192	0.206	1
146	1	14	ARG	CB	C	30.075	0.110	1
147	1	14	ARG	CG	C	26.972	0.176	1
148	1	14	ARG	CD	C	44.166	0.153	1
149	1	14	ARG	N	N	130.114	0.015	1
150	1	15	ASP	H	H	8.259	0.010	1
151	1	15	ASP	HA	H	4.567	0.031	1
152	1	15	ASP	HB2	H	2.479	0.015	2
153	1	15	ASP	HB3	H	2.753	0.056	2
154	1	15	ASP	C	C	176.543	0.000	1
155	1	15	ASP	CA	C	56.685	0.103	1
156	1	15	ASP	CB	C	42.494	0.122	1
157	1	15	ASP	N	N	120.658	0.075	1
158	1	16	MET	H	H	7.585	0.025	1
159	1	16	MET	HA	H	3.951	0.021	1
160	1	16	MET	HB2	H	2.012	0.028	2
161	1	16	MET	HB3	H	2.038	0.000	2
162	1	16	MET	HG2	H	2.440	0.037	2
163	1	16	MET	HG3	H	2.435	0.037	2
164	1	16	MET	HE1	H	1.979	0.029	1
165	1	16	MET	HE2	H	1.979	0.029	1
166	1	16	MET	HE3	H	1.979	0.029	1
167	1	16	MET	C	C	176.720	0.000	1
168	1	16	MET	CA	C	58.000	0.226	1
169	1	16	MET	CB	C	32.518	0.164	1
170	1	16	MET	CG	C	32.052	0.223	1
171	1	16	MET	CE	C	16.773	0.097	1
172	1	16	MET	N	N	118.015	0.074	1
173	1	17	LYS	H	H	7.648	0.004	1
174	1	17	LYS	HA	H	4.222	0.011	1
175	1	17	LYS	HB2	H	1.785	0.014	2
176	1	17	LYS	HB3	H	1.884	0.012	2
177	1	17	LYS	HG2	H	1.378	0.004	2
178	1	17	LYS	HG3	H	1.602	0.012	2
179	1	17	LYS	HD2	H	1.705	0.000	1
180	1	17	LYS	HD3	H	1.705	0.000	1
181	1	17	LYS	HE2	H	2.891	0.000	1
182	1	17	LYS	HE3	H	2.891	0.000	1
183	1	17	LYS	C	C	176.996	0.000	1
184	1	17	LYS	CA	C	56.505	0.184	1
185	1	17	LYS	CB	C	32.315	0.161	1
186	1	17	LYS	CG	C	24.840	0.108	1
187	1	17	LYS	CD	C	28.929	0.000	1
188	1	17	LYS	CE	C	42.105	0.000	1
189	1	17	LYS	N	N	116.980	0.039	1
190	1	18	GLN	H	H	7.527	0.006	1
191	1	18	GLN	HA	H	4.273	0.035	1
192	1	18	GLN	HB2	H	2.049	0.009	2
193	1	18	GLN	HB3	H	1.938	0.021	2
194	1	18	GLN	HG2	H	2.339	0.027	1
195	1	18	GLN	HG3	H	2.339	0.027	1
196	1	18	GLN	HE21	H	7.447	0.002	1
197	1	18	GLN	HE22	H	6.819	0.002	1
198	1	18	GLN	C	C	175.416	0.000	1
199	1	18	GLN	CA	C	55.948	0.239	1
200	1	18	GLN	CB	C	29.337	0.081	1
201	1	18	GLN	CG	C	33.475	0.221	1
202	1	18	GLN	N	N	117.934	0.054	1
203	1	18	GLN	NE2	N	111.753	0.008	1
204	1	19	ARG	H	H	7.905	0.001	1
205	1	19	ARG	HA	H	3.932	0.023	1
206	1	19	ARG	HB2	H	1.792	0.043	2
207	1	19	ARG	HB3	H	1.793	0.046	2
208	1	19	ARG	HG2	H	1.485	0.027	1
209	1	19	ARG	HG3	H	1.485	0.027	1
210	1	19	ARG	HD2	H	3.025	0.004	1
211	1	19	ARG	HD3	H	3.025	0.004	1
212	1	19	ARG	C	C	180.978	0.000	1
213	1	19	ARG	CA	C	58.720	0.180	1
214	1	19	ARG	CB	C	30.256	0.196	1
215	1	19	ARG	CG	C	27.809	0.241	1
216	1	19	ARG	CD	C	43.460	0.140	1
217	1	19	ARG	N	N	127.844	0.008	1
218	1	20	GLY	H	H	8.045	0.354	1
219	1	20	GLY	HA2	H	4.078	0.016	2
220	1	20	GLY	HA3	H	3.752	0.032	2
221	1	20	GLY	C	C	174.419	0.000	1
222	1	20	GLY	CA	C	45.267	0.117	1
223	1	20	GLY	N	N	112.340	0.000	1
224	1	21	GLY	H	H	7.889	0.006	1
225	1	21	GLY	HA2	H	3.823	0.026	2
226	1	21	GLY	HA3	H	3.894	0.018	2
227	1	21	GLY	C	C	171.610	0.000	1
228	1	21	GLY	CA	C	44.294	0.099	1
229	1	21	GLY	N	N	108.204	0.051	1
230	1	22	CYS	H	H	8.234	0.002	1
231	1	22	CYS	HA	H	4.422	0.006	1
232	1	22	CYS	HB2	H	2.553	0.021	2
233	1	22	CYS	HB3	H	2.673	0.017	2
234	1	22	CYS	C	C	176.391	0.000	1
235	1	22	CYS	CA	C	56.460	0.072	1
236	1	22	CYS	CB	C	30.649	0.162	1
237	1	22	CYS	N	N	121.917	0.044	1
238	1	23	PRO	HA	H	4.449	0.018	1
239	1	23	PRO	HB2	H	2.201	0.027	2
240	1	23	PRO	HB3	H	1.973	0.021	2
241	1	23	PRO	HG2	H	1.878	0.039	2
242	1	23	PRO	HG3	H	2.223	0.005	2
243	1	23	PRO	HD2	H	3.995	0.028	2
244	1	23	PRO	HD3	H	3.789	0.009	2
245	1	23	PRO	C	C	177.526	0.000	1
246	1	23	PRO	CA	C	64.152	0.079	1
247	1	23	PRO	CB	C	32.103	0.107	1
248	1	23	PRO	CG	C	26.742	0.070	1
249	1	23	PRO	CD	C	51.477	0.060	1
250	1	24	ARG	H	H	8.556	0.005	1
251	1	24	ARG	HA	H	4.205	0.026	1
252	1	24	ARG	HB2	H	1.576	0.015	2
253	1	24	ARG	HB3	H	1.785	0.020	2
254	1	24	ARG	HG2	H	1.562	0.005	1
255	1	24	ARG	HG3	H	1.562	0.005	1
256	1	24	ARG	HD2	H	3.130	0.015	2
257	1	24	ARG	HD3	H	3.114	0.000	2
258	1	24	ARG	C	C	177.712	0.000	1
259	1	24	ARG	CA	C	57.338	0.000	1
260	1	24	ARG	CB	C	30.873	0.090	1
261	1	24	ARG	CG	C	27.389	0.182	1
262	1	24	ARG	CD	C	43.123	0.193	1
263	1	24	ARG	N	N	120.964	0.055	1
264	1	25	GLY	H	H	7.753	0.005	1
265	1	25	GLY	HA2	H	3.870	0.016	2
266	1	25	GLY	HA3	H	3.871	0.010	2
267	1	25	GLY	C	C	175.844	0.000	1
268	1	25	GLY	CA	C	46.980	0.093	1
269	1	25	GLY	N	N	109.415	0.036	1
270	1	26	ALA	H	H	9.265	0.020	1
271	1	26	ALA	HA	H	4.175	0.017	1
272	1	26	ALA	HB1	H	1.440	0.005	1
273	1	26	ALA	HB2	H	1.440	0.005	1
274	1	26	ALA	HB3	H	1.440	0.005	1
275	1	26	ALA	C	C	178.259	0.000	1
276	1	26	ALA	CA	C	54.024	0.092	1
277	1	26	ALA	CB	C	18.322	0.057	1
278	1	26	ALA	N	N	117.702	0.023	1
279	1	27	SER	H	H	7.967	0.010	1
280	1	27	SER	HA	H	4.431	0.011	1
281	1	27	SER	HB2	H	3.862	0.053	2
282	1	27	SER	HB3	H	3.754	0.021	2
283	1	27	SER	C	C	173.731	0.000	1
284	1	27	SER	CA	C	58.489	0.082	1
285	1	27	SER	CB	C	63.681	0.058	1
286	1	27	SER	N	N	111.688	0.037	1
287	1	28	CYS	H	H	7.499	0.013	1
288	1	28	CYS	HA	H	3.990	0.302	1
289	1	28	CYS	HB2	H	2.728	0.107	2
290	1	28	CYS	HB3	H	2.910	0.150	2
291	1	28	CYS	C	C	176.212	0.000	1
292	1	28	CYS	CA	C	61.387	2.254	1
293	1	28	CYS	CB	C	31.120	0.154	1
294	1	28	CYS	N	N	124.984	0.158	1
295	1	29	THR	H	H	7.979	0.010	1
296	1	29	THR	HA	H	4.132	0.009	1
297	1	29	THR	HB	H	4.363	0.016	1
298	1	29	THR	HG1	H	1.058	0.000	1
299	1	29	THR	HG21	H	1.160	0.028	1
300	1	29	THR	HG22	H	1.160	0.028	1
301	1	29	THR	HG23	H	1.160	0.028	1
302	1	29	THR	C	C	173.058	0.000	1
303	1	29	THR	CA	C	62.075	0.071	1
304	1	29	THR	CB	C	68.600	0.153	1
305	1	29	THR	CG2	C	21.614	0.108	1
306	1	29	THR	N	N	117.211	0.046	1
307	1	30	PHE	H	H	8.974	0.018	1
308	1	30	PHE	HA	H	4.718	0.019	1
309	1	30	PHE	HB2	H	3.065	0.019	2
310	1	30	PHE	HB3	H	3.051	0.012	2
311	1	30	PHE	HD1	H	7.377	0.027	1
312	1	30	PHE	HD2	H	7.355	0.023	1
313	1	30	PHE	HE1	H	7.198	0.023	1
314	1	30	PHE	HE2	H	7.181	0.000	1
315	1	30	PHE	HZ	H	7.300	0.011	1
316	1	30	PHE	C	C	174.780	0.000	1
317	1	30	PHE	CA	C	57.022	0.146	1
318	1	30	PHE	CB	C	39.570	0.070	1
319	1	30	PHE	CD1	C	132.270	0.155	1
320	1	30	PHE	CD2	C	132.270	0.155	1
321	1	30	PHE	CE1	C	130.080	0.000	1
322	1	30	PHE	CE2	C	130.080	0.000	1
323	1	30	PHE	CZ	C	129.233	0.085	1
324	1	30	PHE	N	N	123.748	0.040	1
325	1	31	ALA	H	H	8.891	0.010	1
326	1	31	ALA	HA	H	4.142	0.027	1
327	1	31	ALA	HB1	H	1.385	0.023	1
328	1	31	ALA	HB2	H	1.385	0.023	1
329	1	31	ALA	HB3	H	1.385	0.023	1
330	1	31	ALA	C	C	177.984	0.000	1
331	1	31	ALA	CA	C	52.375	0.084	1
332	1	31	ALA	CB	C	20.013	0.045	1
333	1	31	ALA	N	N	122.717	0.056	1
334	1	32	HIS	H	H	9.261	0.014	1
335	1	32	HIS	HA	H	5.112	0.000	1
336	1	32	HIS	HB2	H	2.955	0.013	2
337	1	32	HIS	HB3	H	3.482	0.012	2
338	1	32	HIS	HD1	H	7.526	0.030	1
339	1	32	HIS	HD2	H	6.798	0.014	1
340	1	32	HIS	HE1	H	7.856	0.074	1
341	1	32	HIS	C	C	173.948	0.000	1
342	1	32	HIS	CA	C	54.896	0.156	1
343	1	32	HIS	CB	C	29.857	0.086	1
344	1	32	HIS	CD2	C	124.844	0.019	1
345	1	32	HIS	CE1	C	139.563	0.055	1
346	1	32	HIS	N	N	117.642	0.048	1
347	1	33	SER	H	H	7.576	0.009	1
348	1	33	SER	HA	H	4.555	0.017	1
349	1	33	SER	HB2	H	4.139	0.029	2
350	1	33	SER	HB3	H	4.217	0.033	2
351	1	33	SER	C	C	173.819	0.000	1
352	1	33	SER	CA	C	57.115	0.142	1
353	1	33	SER	CB	C	65.577	0.074	1
354	1	33	SER	N	N	110.572	0.046	1
355	1	34	GLN	H	H	8.091	0.019	1
356	1	34	GLN	HA	H	3.926	0.043	1
357	1	34	GLN	HB2	H	2.080	0.025	2
358	1	34	GLN	HB3	H	1.995	0.017	2
359	1	34	GLN	HG2	H	2.350	0.027	1
360	1	34	GLN	HG3	H	2.350	0.027	1
361	1	34	GLN	HE21	H	6.656	0.001	1
362	1	34	GLN	HE22	H	7.741	0.003	1
363	1	34	GLN	C	C	177.531	0.020	1
364	1	34	GLN	CA	C	58.934	0.224	1
365	1	34	GLN	CB	C	27.602	0.077	1
366	1	34	GLN	CG	C	33.025	0.090	1
367	1	34	GLN	N	N	119.346	0.146	1
368	1	34	GLN	NE2	N	111.910	0.013	1
369	1	35	GLU	H	H	8.750	0.007	1
370	1	35	GLU	HA	H	3.974	0.032	1
371	1	35	GLU	HB2	H	1.984	0.048	1
372	1	35	GLU	HB3	H	1.984	0.048	1
373	1	35	GLU	HG2	H	2.238	0.011	2
374	1	35	GLU	HG3	H	2.474	0.010	2
375	1	35	GLU	C	C	179.244	0.000	1
376	1	35	GLU	CA	C	59.867	0.176	1
377	1	35	GLU	CB	C	29.149	0.206	1
378	1	35	GLU	CG	C	37.858	0.063	1
379	1	35	GLU	N	N	119.104	0.042	1
380	1	36	GLU	H	H	7.905	0.022	1
381	1	36	GLU	HA	H	3.997	0.035	1
382	1	36	GLU	HB2	H	2.112	0.009	2
383	1	36	GLU	HB3	H	2.115	0.009	2
384	1	36	GLU	HG2	H	2.116	0.000	2
385	1	36	GLU	HG3	H	2.468	0.015	2
386	1	36	GLU	C	C	177.086	0.000	1
387	1	36	GLU	CA	C	59.865	0.109	1
388	1	36	GLU	CB	C	30.970	0.062	1
389	1	36	GLU	CG	C	37.881	0.053	1
390	1	36	GLU	N	N	120.860	0.118	1
391	1	37	LEU	H	H	7.756	0.017	1
392	1	37	LEU	HA	H	4.141	0.008	1
393	1	37	LEU	HB2	H	1.742	0.145	2
394	1	37	LEU	HB3	H	1.623	0.163	2
395	1	37	LEU	HG	H	1.576	0.030	1
396	1	37	LEU	HD11	H	0.670	0.034	2
397	1	37	LEU	HD12	H	0.670	0.034	2
398	1	37	LEU	HD13	H	0.670	0.034	2
399	1	37	LEU	HD21	H	0.732	0.022	2
400	1	37	LEU	HD22	H	0.732	0.022	2
401	1	37	LEU	HD23	H	0.732	0.022	2
402	1	37	LEU	C	C	179.109	0.000	1
403	1	37	LEU	CA	C	57.899	0.080	1
404	1	37	LEU	CB	C	42.452	0.100	1
405	1	37	LEU	CG	C	26.818	0.268	1
406	1	37	LEU	CD1	C	24.994	0.232	2
407	1	37	LEU	CD2	C	25.489	0.090	2
408	1	37	LEU	N	N	120.083	0.128	1
409	1	38	GLU	H	H	8.214	0.007	1
410	1	38	GLU	HA	H	3.898	0.031	1
411	1	38	GLU	HB2	H	1.938	0.076	2
412	1	38	GLU	HB3	H	1.970	0.044	2
413	1	38	GLU	HG2	H	2.263	0.019	2
414	1	38	GLU	HG3	H	2.376	0.017	2
415	1	38	GLU	C	C	178.918	0.000	1
416	1	38	GLU	CA	C	59.049	0.095	1
417	1	38	GLU	CB	C	29.066	0.225	1
418	1	38	GLU	CG	C	36.437	0.165	1
419	1	38	GLU	N	N	116.591	0.040	1
420	1	39	LYS	H	H	7.748	0.010	1
421	1	39	LYS	HA	H	3.848	0.043	1
422	1	39	LYS	HB2	H	1.793	0.023	2
423	1	39	LYS	HB3	H	1.796	0.024	2
424	1	39	LYS	HG2	H	1.433	0.024	1
425	1	39	LYS	HG3	H	1.433	0.024	1
426	1	39	LYS	HD2	H	1.426	0.031	1
427	1	39	LYS	HD3	H	1.426	0.031	1
428	1	39	LYS	HE2	H	2.617	0.010	1
429	1	39	LYS	HE3	H	2.617	0.010	1
430	1	39	LYS	C	C	179.161	0.000	1
431	1	39	LYS	CA	C	59.364	0.186	1
432	1	39	LYS	CB	C	32.308	0.068	1
433	1	39	LYS	CG	C	24.502	0.000	1
434	1	39	LYS	CD	C	29.463	0.045	1
435	1	39	LYS	CE	C	42.008	0.040	1
436	1	39	LYS	N	N	119.971	0.113	1
437	1	40	PHE	H	H	8.173	0.008	1
438	1	40	PHE	HA	H	4.199	0.021	1
439	1	40	PHE	HB2	H	2.997	0.015	2
440	1	40	PHE	HB3	H	2.981	0.021	2
441	1	40	PHE	HD1	H	7.440	0.009	1
442	1	40	PHE	HD2	H	7.440	0.009	1
443	1	40	PHE	HE1	H	7.148	0.000	1
444	1	40	PHE	HE2	H	7.185	0.000	1
445	1	40	PHE	HZ	H	7.311	0.007	1
446	1	40	PHE	C	C	178.725	0.000	1
447	1	40	PHE	CA	C	61.728	0.153	1
448	1	40	PHE	CB	C	38.259	0.065	1
449	1	40	PHE	CD1	C	131.657	0.000	1
450	1	40	PHE	CD2	C	131.657	0.000	1
451	1	40	PHE	CE1	C	130.410	0.000	1
452	1	40	PHE	CE2	C	130.410	0.000	1
453	1	40	PHE	CZ	C	129.520	0.000	1
454	1	40	PHE	N	N	117.405	0.030	1
455	1	41	ARG	H	H	8.471	0.008	1
456	1	41	ARG	HA	H	4.027	0.014	1
457	1	41	ARG	HB2	H	1.828	0.018	2
458	1	41	ARG	HB3	H	1.837	0.009	2
459	1	41	ARG	HG2	H	1.496	0.018	1
460	1	41	ARG	HG3	H	1.496	0.018	1
461	1	41	ARG	HD2	H	3.023	0.000	2
462	1	41	ARG	HD3	H	3.131	0.008	2
463	1	41	ARG	C	C	178.446	0.000	1
464	1	41	ARG	CA	C	59.740	0.092	1
465	1	41	ARG	CB	C	30.241	0.087	1
466	1	41	ARG	CG	C	26.607	0.000	1
467	1	41	ARG	CD	C	43.260	0.087	1
468	1	41	ARG	N	N	120.760	0.034	1
469	1	42	LYS	H	H	7.673	0.005	1
470	1	42	LYS	HA	H	4.048	0.021	1
471	1	42	LYS	HB2	H	1.820	0.033	2
472	1	42	LYS	HB3	H	1.806	0.000	2
473	1	42	LYS	HG2	H	1.476	0.000	1
474	1	42	LYS	HG3	H	1.476	0.000	1
475	1	42	LYS	HD2	H	1.822	0.021	1
476	1	42	LYS	HD3	H	1.822	0.021	1
477	1	42	LYS	HE2	H	2.885	0.000	1
478	1	42	LYS	HE3	H	2.885	0.000	1
479	1	42	LYS	C	C	177.962	0.000	1
480	1	42	LYS	CA	C	58.063	0.096	1
481	1	42	LYS	CB	C	32.363	0.110	1
482	1	42	LYS	CG	C	25.296	0.000	1
483	1	42	LYS	CD	C	29.082	0.280	1
484	1	42	LYS	CE	C	42.089	0.000	1
485	1	42	LYS	N	N	118.270	0.058	1
486	1	43	MET	H	H	7.623	0.015	1
487	1	43	MET	HA	H	4.168	0.012	1
488	1	43	MET	HB2	H	2.015	0.018	2
489	1	43	MET	HB3	H	2.034	0.000	2
490	1	43	MET	HG2	H	2.391	0.000	1
491	1	43	MET	HG3	H	2.391	0.000	1
492	1	43	MET	HE1	H	2.001	0.028	1
493	1	43	MET	HE2	H	2.001	0.028	1
494	1	43	MET	HE3	H	2.001	0.028	1
495	1	43	MET	C	C	176.622	0.000	1
496	1	43	MET	CA	C	57.222	0.198	1
497	1	43	MET	CB	C	32.990	0.250	1
498	1	43	MET	CG	C	31.901	0.000	1
499	1	43	MET	CE	C	16.981	0.110	1
500	1	43	MET	N	N	118.167	0.039	1
501	1	44	ASN	H	H	7.948	0.007	1
502	1	44	ASN	HA	H	4.619	0.017	1
503	1	44	ASN	HB2	H	2.798	0.027	2
504	1	44	ASN	HB3	H	2.706	0.029	2
505	1	44	ASN	HD21	H	7.442	0.002	1
506	1	44	ASN	HD22	H	6.988	0.003	1
507	1	44	ASN	C	C	175.067	0.000	1
508	1	44	ASN	CA	C	53.663	0.076	1
509	1	44	ASN	CB	C	38.953	0.084	1
510	1	44	ASN	N	N	117.870	0.111	1
511	1	44	ASN	ND2	N	112.788	0.022	1
512	1	45	LYS	H	H	7.900	0.015	1
513	1	45	LYS	HA	H	4.211	0.005	1
514	1	45	LYS	HB2	H	1.747	0.000	1
515	1	45	LYS	HB3	H	1.747	0.000	1
516	1	45	LYS	HG2	H	1.353	0.000	1
517	1	45	LYS	HG3	H	1.353	0.000	1
518	1	45	LYS	HD2	H	1.794	0.034	1
519	1	45	LYS	HD3	H	1.794	0.034	1
520	1	45	LYS	HE2	H	2.903	0.000	1
521	1	45	LYS	HE3	H	2.903	0.000	1
522	1	45	LYS	C	C	176.175	0.000	1
523	1	45	LYS	CA	C	56.747	0.003	1
524	1	45	LYS	CB	C	32.671	0.084	1
525	1	45	LYS	CG	C	24.762	0.000	1
526	1	45	LYS	CD	C	28.888	0.000	1
527	1	45	LYS	CE	C	42.124	0.000	1
528	1	45	LYS	N	N	120.840	0.106	1
529	1	46	ARG	H	H	8.119	0.008	1
530	1	46	ARG	HA	H	4.241	0.019	1
531	1	46	ARG	HB2	H	1.724	0.012	2
532	1	46	ARG	HB3	H	1.726	0.000	2
533	1	46	ARG	HG2	H	1.559	0.005	2
534	1	46	ARG	HG3	H	1.542	0.000	2
535	1	46	ARG	HD2	H	3.146	0.028	1
536	1	46	ARG	HD3	H	3.146	0.028	1
537	1	46	ARG	C	C	175.934	0.000	1
538	1	46	ARG	CA	C	56.235	0.059	1
539	1	46	ARG	CB	C	30.623	0.108	1
540	1	46	ARG	CG	C	28.929	0.244	1
541	1	46	ARG	CD	C	43.362	0.000	1
542	1	46	ARG	N	N	121.441	0.046	1
543	1	47	LEU	H	H	8.204	0.003	1
544	1	47	LEU	HA	H	4.343	0.031	1
545	1	47	LEU	HB2	H	1.571	0.029	2
546	1	47	LEU	HB3	H	1.623	0.008	2
547	1	47	LEU	HG	H	1.554	0.000	1
548	1	47	LEU	HD11	H	0.781	0.008	2
549	1	47	LEU	HD12	H	0.781	0.008	2
550	1	47	LEU	HD13	H	0.781	0.008	2
551	1	47	LEU	HD21	H	0.842	0.038	2
552	1	47	LEU	HD22	H	0.842	0.038	2
553	1	47	LEU	HD23	H	0.842	0.038	2
554	1	47	LEU	C	C	176.217	0.000	1
555	1	47	LEU	CA	C	55.339	0.120	1
556	1	47	LEU	CB	C	42.202	0.139	1
557	1	47	LEU	CG	C	26.944	0.108	1
558	1	47	LEU	CD1	C	23.321	0.214	2
559	1	47	LEU	CD2	C	24.909	0.238	2
560	1	47	LEU	N	N	124.072	0.048	1
561	1	48	VAL	H	H	7.484	0.009	1
562	1	48	VAL	HA	H	3.977	0.024	1
563	1	48	VAL	HB	H	1.990	0.014	1
564	1	48	VAL	HG11	H	0.796	0.001	2
565	1	48	VAL	HG12	H	0.796	0.001	2
566	1	48	VAL	HG13	H	0.796	0.001	2
567	1	48	VAL	HG21	H	0.816	0.001	2
568	1	48	VAL	HG22	H	0.816	0.001	2
569	1	48	VAL	HG23	H	0.816	0.001	2
570	1	48	VAL	C	C	180.792	0.000	1
571	1	48	VAL	CA	C	63.409	0.106	1
572	1	48	VAL	CB	C	33.332	0.074	1
573	1	48	VAL	CG1	C	20.025	0.056	2
574	1	48	VAL	CG2	C	21.452	0.058	2
575	1	48	VAL	N	N	124.058	0.131	1

Release date

2023-12-03

Citation

Structure and RNA-binding of the helically extended Roquin CCCH-type zinc finger
Tants, J., Oberstrass, L., Weigand, J., Schlundt, A.
Nucleic Acids Res. (2024), PubMed 38953172 , DOI 10.1093/nar/gkae555 , Abstract

Related entities 1. Znf, entity 1, : 1 : 6 : 83 entities Detail

Interaction partners 1. Znf, entity 1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 1D 1H

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 15N 1H

#	Name	Isotope labeling	Concentration
1	Roquin zinc finger	[U-99% 15N]	360 uM
2	zinc	natural abundance	20 uM
3	sodium chloride	natural abundance	150 mM
4	TRIS	natural abundance	25 mM
5	TCEP	natural abundance	2 mM

2 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 15N 1H

#	Name	Isotope labeling	Concentration
1	Roquin zinc finger	[U-99% 15N]	360 uM
2	zinc	natural abundance	20 uM
3	sodium chloride	natural abundance	150 mM
4	TRIS	natural abundance	25 mM
5	TCEP	natural abundance	2 mM

3 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

4 3D CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

5 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 15N 1H

#	Name	Isotope labeling	Concentration
1	Roquin zinc finger	[U-99% 15N]	360 uM
2	zinc	natural abundance	20 uM
3	sodium chloride	natural abundance	150 mM
4	TRIS	natural abundance	25 mM
5	TCEP	natural abundance	2 mM

6 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

7 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

8 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

9 3D HBHANH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

10 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

12 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 13C 15N 1H

#	Name	Isotope labeling	Concentration
6	Roquin zinc finger	[U-99% 13C; U-99% 15N]	740 uM
7	zinc	natural abundance	20 uM
8	sodium chloride	natural abundance	150 mM
9	TRIS	natural abundance	25 mM
10	TCEP	natural abundance	2 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr52226_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr52226_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	575		100.0 (chain: 1, length: 48)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 48, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 324
  - ¹³C chemical shifts: 208
  - ¹⁵N chemical shifts: 43
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	HIS	HE2	7.339
9	LYS	HZ1	7.346
9	LYS	HZ2	7.346
9	LYS	HZ3	7.346
29	THR	HG1	1.058
32	HIS	HD1	7.526

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	305	286	93.8
¹³C chemical shifts	215	208	96.7
¹⁵N chemical shifts	50	43	86.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	99	91	91.9
¹³C chemical shifts	96	92	95.8
¹⁵N chemical shifts	47	40	85.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	206	195	94.7
¹³C chemical shifts	119	116	97.5
¹⁵N chemical shifts	3	3	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	16	16	100.0
¹³C chemical shifts	16	16	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	22	100.0
¹³C chemical shifts	22	22	100.0

Keywords zinc finger, RNA-binding, DNA-binding, mRNA regulation, immune regulation, Roquin protein

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Found 1 Document (0.544 seconds)

BMRB: 52226

Related entities 1. Znf, entity 1, : 1 : 6 : 83 entities Detail

Interaction partners 1. Znf, entity 1, : 5 interactors Detail

Experiments performed 12 experiments Detail

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Chemical shift validation 4 contents Detail