BMRB: 5240

15N assignment of Alicyclobacillus acidocaldarius thermostable thioredoxin

Authors

Leone, M., Di Lello, P., Pedone, E.MARIA., Bartolucci, S., Rossi, M., Di Blasio, B., Pedone, C., Saviano, M., Isernia, C., Fattorusso, R.

Assembly

Thioredoxin

Entity

1. Thioredoxin (polymer, Thiol state: all disulfide bound), 105 monomers, 11576.21 Da Detail

ATMTLTDANF QQAIQGDKPV LVDFWAAWCG PCRMMAPVLE EFAEAHADKV TVAKLNVDEN PETTSQFGIM SIPTLILFKG GRPVKQLIGY QPKEQLEAQL ADVLQ

Formula weight

11576.21 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS29:SG	1:CYS32:SG

Source organism

Alicyclobacillus acidocaldarius subsp. acidocaldarius

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 99.1 %, Completeness (bb): 99.0 % Detail

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Polymer type: polypeptide(L)

	Total	15N
All	99.1 % (111 of 112)	99.1 % (111 of 112)
Backbone	99.0 % (97 of 98)	99.0 % (97 of 98)
Sidechain	100.0 % (14 of 14)	100.0 % (14 of 14)
Aromatic	100.0 % (2 of 2)	100.0 % (2 of 2)

1. Alicyclobacillus acidocaldarius thioredoxin

ATMTLTDANF QQAIQGDKPV LVDFWAAWCG PCRMMAPVLE EFAEAHADKV TVAKLNVDEN PETTSQFGIM SIPTLILFKG GRPVKQLIGY QPKEQLEAQL ADVLQ

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Sample

Temperature 298.0 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Alicyclobacillus acidocaldarius thioredoxin	[U-15N]		1.0 mM

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Release date

2004-09-09

Citation 1

Solution structure and backbone dynamics of the K18G/R82E Alicyclobacillus acidocaldarius thioredoxin mutant: a molecular analysis of its reduced thermal stability
Leone, M., Di Lello, P., Ohlenschlager, O., Pedone, E.MARIA., Bartolucci, S., Rossi, M., Di Blasio, B., Pedone, C., Saviano, M., Isernia, C., Fattorusso, R.
Biochemistry (2004), 43, 6043-6058, PubMed 15147188 , DOI 10.1021/bi036261d , Abstract

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Citation 2

A single point mutation (Glu85Arg) increases the stability of the thioredoxin from Escherichia coli
Pedone, E., Saviano, M., Rossi, M., Bartolucci, S.
Protein Eng. (2001), 14, 255-260, PubMed 11391017 , DOI: , Abstract

Citation 3

NMR solution structure of a novel thioredoxin from Bacillus acidocaldarius possible determinants of protein stability
Nicastro, G., De Chiara, C., Pedone, E., Tato, M., Rossi, M., Bartolucci, S.
Eur. J. Biochem. (2000), 267, 403-413, PubMed 10632710 , DOI: , Abstract

Citation 4

Thioredoxin from Bacillus acidocaldarius: characterization, high-level expression in Escherichia coli and molecular modelling
Bartolucci, S., Guagliardi, A., Pedone, E., De Pascale, D., Cannio, R., Camardella, L., Rossi, M., Nicastro, G., de Chiara, C., Facci, P., Mascetti, G., Nicolini, C.
Biochem. J. (1997), 328 ( Pt 1), 277-285, PubMed 9359865 , DOI: , Abstract

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Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. Thioredoxin, : 1 : 1 : 3 : 252 entities Detail

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail