A Quick Solution Structure Determination of the Fully Oxidized Double Mutant K9-10A Cytochrome c7 from Desulfuromonas acetoxidans and Mechanistic Implications
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:HIS17:NE2 | 2:HEC1:FE |
2 | na | sing | 1:HIS30:NE2 | 2:HEC1:FE |
3 | na | sing | 1:HIS45:NE2 | 2:HEC1:FE |
4 | na | sing | 1:HIS66:NE2 | 2:HEC1:FE |
5 | na | sing | 1:HIS20:NE2 | 2:HEC1:FE |
6 | na | sing | 1:HIS53:NE2 | 2:HEC1:FE |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 84.1 % (313 of 372) | 84.1 % (313 of 372) |
Backbone | 96.4 % (135 of 140) | 96.4 % (135 of 140) |
Sidechain | 76.7 % (178 of 232) | 76.7 % (178 of 232) |
Aromatic | 14.3 % (3 of 21) | 14.3 % (3 of 21) |
Methyl | 89.7 % (26 of 29) | 89.7 % (26 of 29) |
1. cytochrome c7
ADVVTYENAA GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIKPressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c7 | 2 mM | ||
2 | oxidized heme c | 6 mM | ||
3 | sodium phosphate buffer | 100 mM | ||
4 | D2O | 10 % | ||
5 | H2O | 90 % |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 44.1 % (328 of 744) | 44.1 % (328 of 744) |
Backbone | 52.1 % (146 of 280) | 52.1 % (146 of 280) |
Sidechain | 39.2 % (182 of 464) | 39.2 % (182 of 464) |
Aromatic | 7.1 % (3 of 42) | 7.1 % (3 of 42) |
Methyl | 46.6 % (27 of 58) | 46.6 % (27 of 58) |
1. cytochrome c7
ADVVTYENAA GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIKPressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c7 | 2 mM | ||
2 | oxidized heme c | 6 mM | ||
3 | sodium phosphate buffer | 100 mM | ||
4 | D2O | 10 % | ||
5 | H2O | 90 % |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 29.9 % (334 of 1116) | 29.9 % (334 of 1116) |
Backbone | 36.0 % (151 of 420) | 36.0 % (151 of 420) |
Sidechain | 26.3 % (183 of 696) | 26.3 % (183 of 696) |
Aromatic | 4.8 % (3 of 63) | 4.8 % (3 of 63) |
Methyl | 32.2 % (28 of 87) | 32.2 % (28 of 87) |
1. cytochrome c7
ADVVTYENAA GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIKPressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c7 | 2 mM | ||
2 | oxidized heme c | 6 mM | ||
3 | sodium phosphate buffer | 100 mM | ||
4 | D2O | 10 % | ||
5 | H2O | 90 % |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 23.3 % (346 of 1488) | 23.3 % (346 of 1488) |
Backbone | 28.6 % (160 of 560) | 28.6 % (160 of 560) |
Sidechain | 20.0 % (186 of 928) | 20.0 % (186 of 928) |
Aromatic | 3.6 % (3 of 84) | 3.6 % (3 of 84) |
Methyl | 25.0 % (29 of 116) | 25.0 % (29 of 116) |
1. cytochrome c7
ADVVTYENAA GNVTFDHKAH AEKLGCDACH EGTPAKIAID KKSAHKDACK TCHKSNNGPT KCGGCHIKPressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c7 | 2 mM | ||
2 | oxidized heme c | 6 mM | ||
3 | sodium phosphate buffer | 100 mM | ||
4 | D2O | 10 % | ||
5 | H2O | 90 % |