BMRBj - BMREntryMaster

Found 1 Document (1.767 seconds)

BMRB: 5288

1MW4

Assignment of Backbone 1H, 13C, and 15N Resonances of Human Grb7-SH2 Domain in Complex with a Phosphorylated Peptide Ligand

Authors

Brescoa, P.J., Ivancic, M., Lyons, B.A.

Assembly

Grb7 SH2 domain / phosphorylated peptide complex

Entity

1. growth factor receptor bound protein 7 (polymer, Thiol state: not reported), 120 monomers, 13671.55 Da Detail

GSPASGTSLS AAIHRTQLWF HGRISREESQ RLIGQQGLVD GLFLVRESQR NPQGFVLSLC HLQKVKHYLI LPSEEEGRLY FSMDDGQTRF TDLLQLVEFH QLNRGILPCL LRHCCTRVAL

2. phosphorylated peptide, pY1139 (polymer, Thiol state: not reported), 1 monomers, 261.168 Da

Total weight

13932.718 Da

Max. entity weight

13671.55 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 35.0 %, Completeness (bb): 62.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	35.0 % (498 of 1424)	22.1 % (164 of 743)	39.7 % (219 of 552)	89.1 % (115 of 129)
Backbone	62.2 % (443 of 712)	49.6 % (122 of 246)	59.1 % (207 of 350)	98.3 % (114 of 116)
Sidechain	16.8 % (138 of 822)	8.5 % (42 of 497)	30.4 % (95 of 312)	7.7 % (1 of 13)
Aromatic	3.6 % (4 of 112)	3.6 % (2 of 56)	3.6 % (2 of 55)	0.0 % (0 of 1)
Methyl	8.5 % (12 of 142)	5.6 % (4 of 71)	11.3 % (8 of 71)

1. growth factor receptor bound protein 7

GSPASGTSLS AAIHRTQLWF HGRISREESQ RLIGQQGLVD GLFLVRESQR NPQGFVLSLC HLQKVKHYLI LPSEEEGRLY FSMDDGQTRF TDLLQLVEFH QLNRGILPCL LRHCCTRVAL

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	growth factor receptor bound protein 7	[U-98% 13C; U-98% 15N]		0.8 mM
3	phosphorylated peptide, pY1139			0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Release date

2002-08-22

Citation

Assignment of backbone 1H, 13C, and 15N resonances of human Grb7-SH2 domain in complex with a phosphorylated peptide ligand
Brescoa, P.J., Ivancic, M., Lyons, B.A.
J. Biomol. NMR (2002), 23, 77-78, PubMed 12061724 , Abstract

Related entities 1. growth factor receptor bound protein 7, : 1 : 10 : 4 : 108 entities Detail

Interaction partners 1. growth factor receptor bound protein 7, : 45 interactors Detail

More details for 45 interactors identified by 19 citations

Experiments performed 5 experiments Detail

#	Name	Isotope labeling	Type	Concentration
1	growth factor receptor bound protein 7	[U-98% 13C; U-98% 15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	growth factor receptor bound protein 7	[U-98% 13C; U-98% 15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	growth factor receptor bound protein 7	[U-98% 13C; U-98% 15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	growth factor receptor bound protein 7	[U-98% 13C; U-98% 15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	growth factor receptor bound protein 7	[U-98% 13C; U-98% 15N]		0.8 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5288_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5288_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	426		98.3 (chain: 1, length: 120)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 120, Sequence coverage: 98.3%
- Total number of assignments
  - ¹³C chemical shifts: 198
  - ¹H chemical shifts: 114
  - ¹⁵N chemical shifts: 114
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	552	198	35.9
¹H chemical shifts	743	114	15.3
¹⁵N chemical shifts	140	114	81.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	240	117	48.7
¹H chemical shifts	246	114	46.3
¹⁵N chemical shifts	116	114	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	312	81	26.0
¹H chemical shifts	497	0	0.0
¹⁵N chemical shifts	24	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	72	4	5.6
¹H chemical shifts	72	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	55	0	0.0
¹H chemical shifts	56	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords Cell Signaling, Growth Factor Receptor Bound, NMR assignments, Protein, Src homology 2

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Found 1 Document (1.767 seconds)

BMRB: 5288

Sample

Chemical shift reference

Chemical shift reference

Related entities 1. growth factor receptor bound protein 7, : 1 : 10 : 4 : 108 entities Detail

Interaction partners 1. growth factor receptor bound protein 7, : 45 interactors Detail

Experiments performed 5 experiments Detail

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Chemical shift validation 4 contents Detail