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Found 1 Document (1.644 seconds)

BMRB: 5334

1G03

Backbone 1H, 13C, and 15N resonance assignments and secondary structure of the D54A mutant of HTLV-I capsid protein

Authors

Cornilescu, C.C., Bouamr, F., Carter, C., Tjandra, N.

Assembly

NTD HTLV-I capsid protein

Entity

1. NTD HTLV-I capsid protein (polymer, Thiol state: all free), 134 monomers, 14841.66 Da Detail

PVMHPHGAPP NHRPWQMKDL QAIKQEVSQA APGSPQFMQT IRLAVQQFDP TAKALQDLLQ YLCSSLVASL HHQQLDSLIS EAETRGITSY NPLAGPLRVQ ANNPQQQGLR REYQQLWLAA FAALPGSAKD PSWA

Formula weight

14841.66 Da

Source organism

Human T-cell leukemia virus type I

Exptl. method

NMR

Refine. method

distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 59.3 %, Completeness (bb): 91.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	59.3 % (931 of 1569)	53.1 % (437 of 823)	62.7 % (376 of 600)	80.8 % (118 of 146)
Backbone	91.5 % (712 of 778)	88.9 % (232 of 261)	91.7 % (363 of 396)	96.7 % (117 of 121)
Sidechain	37.2 % (342 of 919)	36.5 % (205 of 562)	41.0 % (136 of 332)	4.0 % (1 of 25)
Aromatic	0.0 % (0 of 110)	0.0 % (0 of 55)	0.0 % (0 of 52)	0.0 % (0 of 3)
Methyl	33.8 % (48 of 142)	32.4 % (23 of 71)	35.2 % (25 of 71)

1. Human T-cell Leukemia Virus type I

PVMHPHGAPP NHRPWQMKDL QAIKQEVSQA APGSPQFMQT IRLAVQQFDP TAKALQDLLQ YLCSSLVASL HHQQLDSLIS EAETRGITSY NPLAGPLRVQ ANNPQQQGLR REYQQLWLAA FAALPGSAKD PSWA

Show sample condition

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.19 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.24 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1G03, Strand ID: A Detail

Release date

2003-06-09

Citation 1

Backbone 1H, 13C, and 15N resonance assignments and secondary structure of the D54A mutant of HTLV-I capsid protein
Cornilescu, C.C., Bouamr, F., Carter, C., Tjandra, N.

Show more 4 citaions

Citation 2

Structural analysis of the N-terminal domain of the human T-cell leukemia virus capsid protein
Cornilescu, C.C., Bouamr, F., Yao, X., Carter, C., Tjandra, N.
J. Mol. Biol. (2001), 306, 783-797, PubMed 11243788 , DOI 10.1006/jmbi.2000.4395 , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 4

Protein backbone angle restraints from searching a database for chemical shift and sequence homology
Cornilescu, G., Delaglio, F., Bax, A.
J. Biomol. NMR (1999), 13, 289-302, PubMed 10212987 , Abstract

Citation 5

A short recollection on the paper entitled "A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams" by D.S. Garrett, R. Powers, A.M. Gronenborn, and G.M. Clore [J. Magn. Reson. 95 (1991) 214-220]
J. Magn. Reson. (2011), 213, 364-365, PubMed 22152356 , DOI 10.1016/j.jmr.2011.08.009 , Abstract

Hide non-primary 4 citaions

Related entities 1. NTD HTLV-I capsid protein, : 1 : 23 : 21 entities Detail

Interaction partners 1. NTD HTLV-I capsid protein, : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 1H-15N HSQC

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

2 CBCACONH

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

3 HNCACB

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

4 HBHACONH

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

5 3D HNCO

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

6 3D 1H-15N NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

7 1H-13C CT-HSQC

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human T-cell Leukemia Virus type I	[U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Human T-cell Leukemia Virus type I	[U-13C; U-15N]		0.8 ~ 1.0 mM

nullKeywords HTLV-I, mutant, retrovirus, capsid protein

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Found 1 Document (1.644 seconds)

BMRB: 5334

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. NTD HTLV-I capsid protein, : 1 : 23 : 21 entities Detail

Interaction partners 1. NTD HTLV-I capsid protein, : 6 interactors Detail

Experiments performed 7 experiments Detail

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