BMRBj - BMREntryMaster

Found 1 Document (1.84 seconds)

BMRB: 5335

1LV3

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for E.coli protein YacG

Authors

Ramelot, T.A., Kennedy, M.A.

Assembly

YacG

Entity

1. YacG (polymer, Thiol state: all other bound), 68 monomers, 7587.361 Da Detail

GSHMSETITV NCPTCGKTVV WGEISPFRPF CSKRCQLIDL GEWAAEEKRI PSSGDLSESD DWSEEPKQ

2. ZN (non-polymer), 65.409 Da

Total weight

7652.77 Da

Max. entity weight

7587.361 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:CYS31:SG	2:ZN1:ZN
4	na	sing	1:CYS35:SG	2:ZN1:ZN

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 86.2 %, Completeness (bb): 91.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (662 of 768)	85.1 % (342 of 402)	86.9 % (258 of 297)	89.9 % (62 of 69)
Backbone	91.2 % (363 of 398)	91.2 % (124 of 136)	91.0 % (181 of 199)	92.1 % (58 of 63)
Sidechain	82.9 % (359 of 433)	82.0 % (218 of 266)	85.1 % (137 of 161)	66.7 % (4 of 6)
Aromatic	78.3 % (47 of 60)	83.3 % (25 of 30)	70.4 % (19 of 27)	100.0 % (3 of 3)
Methyl	88.5 % (46 of 52)	84.6 % (22 of 26)	92.3 % (24 of 26)

1. YacG

GSHMSETITV NCPTCGKTVV WGEISPFRPF CSKRCQLIDL GEWAAEEKRI PSSGDLSESD DWSEEPKQ

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1LV3, Strand ID: A Detail

Release date

2002-09-08

Citation

NMR structure of the Escherichia coli protein YacG: a novel sequence motif in the zinc-finger family of proteins
Ramelot, T.A., Cort, J.R., Yee, A.A., Semesi, A., Edwards, A.M., Arrowsmith, C.H., Kennedy, M.A.
Proteins (2002), 49, 289-293, PubMed 12211008 , DOI 10.1002/prot.10214 , Abstract

Related entities 1. YacG, : 1 : 5 : 48 entities Detail

Interaction partners 1. YacG, : 4 interactors Detail

More details for 4 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

2 1H-13C HSQC (aliph)

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

3 15N-edited NOESY-HQSC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

4 HCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

5 HCCH-TOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

6 CN-NOESY-HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

7 HNCO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

8 HNCACB

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

9 CBCA(CO)NH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

10 CCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

11 HNHA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

12 4D CC-NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

13 1H-13C HSQC (alpha)

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

14 1H-13C HSQC (arom)

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

null

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Found 1 Document (1.84 seconds)

BMRB: 5335

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Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. YacG, : 1 : 5 : 48 entities Detail

Interaction partners 1. YacG, : 4 interactors Detail

Experiments performed 14 experiments Detail

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