BMRBj - BMREntryMaster

Found 1 Document (1.572 seconds)

BMRB: 5343

2G0L

1H, 13C, 15N Assignment of Neocarzinostatin Apo-Protein

Authors

Muskett, F.W., Stoneman, R., Caddick, S., Woolfson, D.N.

Assembly

Neocarzinostatin apo-protein

Entity

1. Neocarzinostatin apo-protein (polymer, Thiol state: all disulfide bound), 122 monomers, 12217.18 Da Detail

HHHHHHLQGA APTATVTPSS GLSDGTVVKV AGAGLQAGTA YDVGQCAWVD TGVLACNPAD FSSVTADANG SASTSLTVRR SFEGFLFDGT RWGTVDCTTA ACQVGLSDAA GNGPEGVAIS FN

Formula weight

12217.18 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS46:SG	1:CYS56:SG
2	disulfide	sing	1:CYS97:SG	1:CYS102:SG

Source organism

Streptomyces carzinostaticus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 83.4 %, Completeness (bb): 91.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.4 % (1015 of 1217)	83.1 % (502 of 604)	81.6 % (396 of 485)	91.4 % (117 of 128)
Backbone	91.0 % (659 of 724)	89.8 % (230 of 256)	91.4 % (320 of 350)	92.4 % (109 of 118)
Sidechain	75.8 % (454 of 599)	78.2 % (272 of 348)	72.2 % (174 of 241)	80.0 % (8 of 10)
Aromatic	29.2 % (31 of 106)	45.3 % (24 of 53)	9.8 % (5 of 51)	100.0 % (2 of 2)
Methyl	95.1 % (135 of 142)	97.2 % (69 of 71)	93.0 % (66 of 71)

1. Neocarzinostatin

HHHHHHLQGA APTATVTPSS GLSDGTVVKV AGAGLQAGTA YDVGQCAWVD TGVLACNPAD FSSVTADANG SASTSLTVRR SFEGFLFDGT RWGTVDCTTA ACQVGLSDAA GNGPEGVAIS FN

Show sample condition

Sample

Temperature 308 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Neocarzinostatin	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	sodium azide		0.005 % w/w

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.63 ppm, Outliers: 1 Detail

Release date

2002-09-04

Citation 1

Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore
Urbaniak, M.D., Muskett, F.W., Finucane, M.D., Caddick, S., Woolfson, D.N.
Biochemistry (2002), 41, 11731-11739, PubMed 12269815 , Abstract

Show more 2 citaions

Citation 2

To be announced
Stoneman, R., Muskett, F.W., Caddick, S., Woolfson, D.N.
J. Am. Chem. Soc.

Citation 3

The amino acid sequence of neocarzinostatin apoprotein deduced from the base sequence of the gene
Sakata, N., Minamitani, S., Kanbe, T., Hori, M., Hamada, M., Edo, K.
Biol. Pharm. Bull. (1993), 16, 26-28, PubMed 8369747 , DOI 10.1248/bpb.16.26 , Abstract

Hide non-primary 2 citaions

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 6888 released on 2007-02-05
    Title 1H, 15N Assignment of Neocarzinostatin Apo-Protein complexed with Flavone
  BMRB: 5344 released on 2002-09-04
    Title 1H, 15N Assignment of Neocarzinostatin Apo-Protein Bound to a Synthetic Chromophore
  Swiss-Prot: P0A3R9 released on 1986-07-21
    Title NCZS_STRCZ Entity Neocarzinostatin

Related entities 1. Neocarzinostatin apo-protein, : 1 : 3 : 9 : 3 entities Detail

Experiments performed 11 experiments Detail

1 1H15N HSQC