1H, 13C, 15N Assignment of Neocarzinostatin Apo-Protein
HHHHHHLQGA APTATVTPSS GLSDGTVVKV AGAGLQAGTA YDVGQCAWVD TGVLACNPAD FSSVTADANG SASTSLTVRR SFEGFLFDGT RWGTVDCTTA ACQVGLSDAA GNGPEGVAIS FN
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS46:SG | 1:CYS56:SG |
2 | disulfide | sing | 1:CYS97:SG | 1:CYS102:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.4 % (1015 of 1217) | 83.1 % (502 of 604) | 81.6 % (396 of 485) | 91.4 % (117 of 128) |
Backbone | 91.0 % (659 of 724) | 89.8 % (230 of 256) | 91.4 % (320 of 350) | 92.4 % (109 of 118) |
Sidechain | 75.8 % (454 of 599) | 78.2 % (272 of 348) | 72.2 % (174 of 241) | 80.0 % (8 of 10) |
Aromatic | 29.2 % (31 of 106) | 45.3 % (24 of 53) | 9.8 % (5 of 51) | 100.0 % (2 of 2) |
Methyl | 95.1 % (135 of 142) | 97.2 % (69 of 71) | 93.0 % (66 of 71) |
1. Neocarzinostatin
HHHHHHLQGA APTATVTPSS GLSDGTVVKV AGAGLQAGTA YDVGQCAWVD TGVLACNPAD FSSVTADANG SASTSLTVRR SFEGFLFDGT RWGTVDCTTA ACQVGLSDAA GNGPEGVAIS FNTemperature 308 (±0.5) K, pH 5.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Neocarzinostatin | [U-13C; U-15N] | 1 mM | |
2 | phosphate buffer | 25 mM | ||
3 | sodium azide | 0.005 % w/w |