Solution structure of ascidian trypsin inhibitor determined by nuclear magnetic resonance spectroscopy
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS5:SG | 1:CYS40:SG |
2 | disulfide | sing | 1:CYS12:SG | 1:CYS41:SG |
3 | disulfide | sing | 1:CYS14:SG | 1:CYS37:SG |
4 | disulfide | sing | 1:CYS23:SG | 1:CYS54:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 98.4 % (305 of 310) | 98.4 % (305 of 310) |
Backbone | 99.1 % (111 of 112) | 99.1 % (111 of 112) |
Sidechain | 98.0 % (194 of 198) | 98.0 % (194 of 198) |
Aromatic | 95.0 % (19 of 20) | 95.0 % (19 of 20) |
Methyl | 100.0 % (19 of 19) | 100.0 % (19 of 19) |
1. ascidian trypsin inhibitor
AHMDCTEFNP LCRCNKMLGD LICAVIGDAK EEHRNMCALC CEHPGGFEYS NGPCETemperature 303 (±1) K, pH 3.7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ascidian trypsin inhibitor | 1.5 ~ 2.0 mM |