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BMRB: 5379


5379
1LJV
Bovine Pancreatic Polypeptide Bound to DPC Micelles
Authors
Lerch, M.S., Gafner, V., Bader, R.U., Christen, B., Zerbe, O.
Assembly
PANCREATIC POLYPEPTIDE
Entity
1. PANCREATIC POLYPEPTIDE (polymer, Thiol state: not present), 37 monomers, 4224.710 Da Detail

APLEPEYPGD NATPEQMAQY AAELRRYINM LTRPRYX


Formula weight
4224.71 Da
Source organism
Bos taurus
Exptl. method
NMR
Refine. method
simulated annealing restraint energy minimization with explicit water
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.3 %, Completeness: 95.8 %, Completeness (bb): 92.9 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All95.8 % (251 of 262)96.5 % (219 of 227)91.4 % (32 of 35)
Backbone92.9 % (92 of 99)92.6 % (63 of 68)93.5 % (29 of 31)
Sidechain91.4 % (149 of 163)92.5 % (147 of 159)50.0 % (2 of 4)
Aromatic100.0 % (16 of 16)100.0 % (16 of 16)
Methyl100.0 % (15 of 15)100.0 % (15 of 15)

1. Pancreatic polypeptide

APLEPEYPGD NATPEQMAQY AAELRRYINM LTRPRYX

Show sample condition
Sample

Pressure 1 atm, Temperature 310 (±0.01) K


#NameIsotope labelingTypeConcentration
1Pancreatic polypeptide[U-95% 15N]3 mM
2DPC-d38300 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1LJV, Strand ID: A Detail

Release date
2003-02-19
Citation 1
Bovine pancreatic polypeptide (bPP) undergoes significant changes in conformation and dynamics upon binding to DPC micelles
Lerch, M.S., Gafner, V., Bader, R.U., Christen, B., Folkers, G., Zerbe, O.
J. Mol. Biol. (2002), 322, 1117-1133, PubMed 12367532 , DOI 10.1016/s0022-2836(02)00889-6 ,
Show more 2 citaions
Citation 2
The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 ,
Citation 3
Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 ,
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Related entities 1. PANCREATIC POLYPEPTIDE, : 1 : 6 : 111 entities Detail
More details for 118 related entities
Experiments performed 2 experiments Detail
nullKeywords NMR micelle peptide hormone