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1H, 13C and 15N assignments for the cold shock response protein Yfia
Authors
Kalinin, A., Rak, A., Shcherbakov, D., Bayer, P.
Assembly
Yfia
Entity
1. Yfia (polymer, Thiol state: not present), 113 monomers, 12784.42 Da Detail

MTMNITSKQM EITPAIRQHV ADRLAKLEKW QTHLINPHII LSKEPQGFVA DATINTPNGV LVASGKHEDM YTAINELINK LERQLNKLQH KGEARRAATS VKDANFVEEV EEE


Formula weight
12784.42 Da
Source organism
Escherichia coli
Exptl. method
NMR
Refine. method
Torsion angle dynamics and simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 95.2 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All95.2 % (1266 of 1330)96.3 % (669 of 695)93.9 % (480 of 511)94.4 % (117 of 124)
Backbone95.8 % (642 of 670)96.9 % (219 of 226)95.2 % (319 of 335)95.4 % (104 of 109)
Sidechain95.1 % (731 of 769)95.9 % (450 of 469)94.0 % (268 of 285)86.7 % (13 of 15)
Aromatic96.7 % (58 of 60)100.0 % (30 of 30)96.6 % (28 of 29) 0.0 % (0 of 1)
Methyl97.1 % (134 of 138)100.0 % (69 of 69)94.2 % (65 of 69)

1. cold shock response protein Yfia

MTMNITSKQM EITPAIRQHV ADRLAKLEKW QTHLINPHII LSKEPQGFVA DATINTPNGV LVASGKHEDM YTAINELINK LERQLNKLQH KGEARRAATS VKDANFVEEV EEE

Sample

Temperature 300 (±0.2) K, pH 6.65 (±0.15)


#NameIsotope labelingTypeConcentration
1cold shock response protein Yfia[U-13C; U-15N]0.5 ~ 0.8 mM

LACS Plot; CA
Referencing offset: 0.13 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.13 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 0.46 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 29 models in PDB: 1N3G, Strand ID: A Detail


Release date
2002-09-24
Citation
1H, 13C and 15N resonance assignments of the ribosome-associated cold shock response protein Yfia of Escherichia coli
Kalinin, A., Rak, A., Shcherbakov, D., Bayer, P.
J. Biomol. NMR (2002), 23, 335-336, PubMed 12398359 , DOI: ,
Related entities 1. Yfia, : 1 : 24 : 4 : 30 entities Detail
Experiments performed 14 experiments Detail
nullKeywords cold shock protein