BMRB: 5395

Chemical Shift Assignments for the Unmodified Branch Site Helix

Authors

Newby, M.I., Greenbaum, N.L.

Assembly

Unmodified U2 snRNA - Intron duplex

Entity

1. U2 snRNA - intron duplex (polymer, Thiol state: not present), 9 monomers, 2975.747 Da Detail

GGUGUAGUA

2. Intron branch site region (polymer, Thiol state: not present), 10 monomers, 3167.896 Da Detail

UACUAACACC

Total weight

6143.643 Da

Max. entity weight

3167.896 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 47.4 %, Completeness: 26.1 %, Completeness (bb): 21.9 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	26.1 % (43 of 165)	26.1 % (43 of 165)
Suger, PO4	21.9 % (25 of 114)	21.9 % (25 of 114)
Nucleobase	35.3 % (18 of 51)	35.3 % (18 of 51)
Aromatic	41.9 % (18 of 43)	41.9 % (18 of 43)

1. U2 snRNA - intron duplex

GGUGUAGUA

2. Intron branch site region

UACUAACACC

Show sample condition

Sample

Pressure 1 atm, Temperature 278 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	U2 snRNA - intron duplex			1.0 mM
2	Intron branch site region			1.0 mM
3	sodium phosphate			10 mM
4	D2O			10 %
5	H2O			90 %
6	EDTA			0.1 mM

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Chem. Shift Complete2

Sequence coverage: 52.6 %, Completeness: 28.2 %, Completeness (bb): 24.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
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Polymer type: polyribonucleotide

	Total	1H
All	28.2 % (93 of 330)	28.2 % (93 of 330)
Suger, PO4	24.1 % (55 of 228)	24.1 % (55 of 228)
Nucleobase	37.3 % (38 of 102)	37.3 % (38 of 102)
Aromatic	44.2 % (38 of 86)	44.2 % (38 of 86)

1. U2 snRNA - intron duplex

GGUGUAGUA

2. Intron branch site region

UACUAACACC

Show sample condition

Sample

Pressure 1 atm, Temperature 278 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	U2 snRNA - intron duplex			1.0 mM
2	Intron branch site region			1.0 mM
3	sodium phosphate			10 mM
4	D2O			10 %
5	H2O			90 %
6	EDTA			0.1 mM

Hide sample codition

Release date

2003-02-19

Citation

Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine
Newby, M.I., Greenbaum, N.L.
Nat. Struct. Biol. (2002), 9, 958-965, PubMed 12426583 , DOI 10.1038/nsb873 , Abstract

Experiments performed 3 experiments Detail