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BMRB: 5408

1IYR

1H, 13C, and 15N Chemical Shift Assignments for DFF-C domain of DFF45/ICAD

Authors

Fukushima, K., Kikuchi, J., Koshiba, S., Kigawa, T., Kuroda, Y., Yokoyama, S.

Assembly

DFF40/DFF45 heterodimer

Entity

1. DFF40/DFF45 heterodimer (polymer, Thiol state: all free), 107 monomers, 11715.02 Da Detail

TGISRETSSD VALASHILTA LREKQAPELS LSSQDLELVT KEDPKALAVA LNWDIKKTET VQEACERELA LRLQQTQSLH SLRSISASKA SPPGDLQNPK RARQDPT

Formula weight

11715.02 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.5 %, Completeness: 35.3 %, Completeness (bb): 65.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	35.3 % (428 of 1211)	27.1 % (172 of 634)	37.6 % (175 of 465)	72.3 % (81 of 112)
Backbone	65.9 % (415 of 630)	78.6 % (165 of 210)	53.0 % (169 of 319)	80.2 % (81 of 101)
Sidechain	3.4 % (23 of 686)	1.7 % (7 of 424)	6.4 % (16 of 251)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 20)	0.0 % (0 of 10)	0.0 % (0 of 9)	0.0 % (0 of 1)
Methyl	6.8 % (9 of 132)	3.0 % (2 of 66)	10.6 % (7 of 66)

1. DNA flagmentation factor 45kDa

TGISRETSSD VALASHILTA LREKQAPELS LSSQDLELVT KEDPKALAVA LNWDIKKTET VQEACERELA LRLQQTQSLH SLRSISASKA SPPGDLQNPK RARQDPT

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

Release date

2003-02-24

Citation

Solution structure of the DFF-C domain of DFF45/ICAD. A structural basis for the regulation of apoptotic DNA fragmentation
Fukushima, K., Kikuchi, J., Koshiba, S., Kigawa, T., Kuroda, Y., Yokoyama, S.
J. Mol. Biol. (2002), 321, 317-327, PubMed 12144788 , DOI: , Abstract

Related entities 1. DFF40/DFF45 heterodimer, : 1 : 2 : 4 entities Detail

Interaction partners 1. DFF40/DFF45 heterodimer, : 25 interactors Detail

More details for 25 interactors identified by 12 citations

Experiments performed 12 experiments Detail

1 15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

2 15N NOESY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

3 15N TOCSY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

4 13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

5 13C NOESY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

6 HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

7 HCCH-COSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

8 HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

9 HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

10 HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

11 CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

12 CBCANH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA flagmentation factor 45kDa			0.5 ~ 3.0 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5408_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5408_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	DFF-C_shift_set	Warning	400		77.6 (chain: 1, length: 107)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: DFF-C_shift_set
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 107, Sequence coverage: 77.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 80
  - ¹H chemical shifts: 163
  - ¹³C chemical shifts: 157
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords apoptosis, DFF40/CAD, chaperone-like activity, stable domain