BMRB: 5457

Complete 1H Chemical Shift Assignment of a 24-residue Sin Interacting Domain of hMad1

Authors

van Ingen, H., Tessari, M., Vuister, G.G.W.

Assembly

complex of hMad1-Sin Interacting Domain (SID) and PAH2 domain of mSin3B

Entity

1. Mad1 (polymer, Thiol state: not present), 24 monomers, 2973.345 Da Detail

VRMNIQMLLE AADYLERRER EAEH

2. mSin3B (polymer, Thiol state: not present), 105 monomers, 12193.36 Da Detail

ESDSVEFNNA ISYVNKIKTR FLDHPEIYRS FLEILHTYQK EQLHTKGRPF RGMSEEEVFT EVANLFRGQE DLLSEFGQFL PEAKRSLFTG NGSAEMNSGQ KNEEK

Total weight

15166.705 Da

Max. entity weight

12193.36 Da

Source organism

Mus musculus , Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING, ITERATIVE NOE-ASSIGNMENT

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 20.2 %, Completeness: 17.8 %, Completeness (bb): 17.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	17.8 % (146 of 820)	17.8 % (146 of 820)
Backbone	17.6 % (46 of 262)	17.6 % (46 of 262)
Sidechain	17.9 % (100 of 558)	17.9 % (100 of 558)
Aromatic	8.7 % (6 of 69)	8.7 % (6 of 69)
Methyl	21.4 % (12 of 56)	21.4 % (12 of 56)

1. Mad1

VRMNIQMLLE AADYLERRER EAEH

2. mSin3B

ESDSVEFNNA ISYVNKIKTR FLDHPEIYRS FLEILHTYQK EQLHTKGRPF RGMSEEEVFT EVANLFRGQE DLLSEFGQFL PEAKRSLFTG NGSAEMNSGQ KNEEK

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 6.3 (±0.1), Details The sample also contained trace amounts of sodiumazide and protease inhibitor

#	Name	Isotope labeling	Type	Concentration
1	Mad1	[U-15N; U-13C]		1.3 mM
2	mSin3B			1.3 mM
3	potassium phosphate			50 mM

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Calculated from 20 models in PDB: 1E91, Strand ID: A, B Detail

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Release date

2002-11-04

Citation

A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment
van Ingen, H., Tessari, M., Vuister, G.G.W.
J. Biomol. NMR (2002), 24, 155-160, PubMed 12495032 , Abstract

Related entities 1. Mad1, : 3 : 24 entities Detail

Related entities 2. mSin3B, : 3 : 32 entities Detail

Interaction partners 1. Mad1, : 6 : 1 interactors Detail

Interaction partners 2. mSin3B, : 9 interactors Detail

Experiments performed 3 experiments Detail