BMRB: 5495

1H chemical shift assignments for Psalmotoxin 1

Authors

Escoubas, P., Bernard, C., Lambeau, G., Lazdunski, M., Darbon, H.

Assembly

psalmotoxin1

Entity

1. psalmotoxin1 (polymer, Thiol state: all disulfide bound), 40 monomers, 4695.431 Da Detail

EDCIPKWKGC VNRHGDCCEG LECWKRRRSF EVCVPKTPKT

Formula weight

4695.431 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS3:SG	1:CYS18:SG
2	disulfide	sing	0:CYS10:SG	1:CYS23:SG
3	disulfide	sing	0:CYS17:SG	1:CYS33:SG

Source organism

Psalmopoeus cambridgei

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 63.4 %, Completeness (bb): 95.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	63.4 % (161 of 254)	63.4 % (161 of 254)
Backbone	95.0 % (76 of 80)	95.0 % (76 of 80)
Sidechain	49.4 % (86 of 174)	49.4 % (86 of 174)
Aromatic	52.6 % (10 of 19)	52.6 % (10 of 19)
Methyl	100.0 % (12 of 12)	100.0 % (12 of 12)

1. psalmotoxin 1

EDCIPKWKGC VNRHGDCCEG LECWKRRRSF EVCVPKTPKT

Show sample condition

Sample

Temperature 300 K

#	Name	Isotope labeling	Type	Concentration
1	psalmotoxin 1			2.9 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1LMM, Strand ID: A Detail

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Release date

2003-07-29

Citation

Recombinant production and solution structure of PcTx1, the specific peptide inhibitor of ASIC1a proton-gated cation channels
Escoubas, P., Bernard, C., Lambeau, G., Lazdunski, M., Darbon, H.
Protein Sci. (2003), 12, 1332-1343, PubMed 12824480 , DOI 10.1110/ps.0307003 , Abstract

Related entities 1. psalmotoxin1, : 1 : 4 : 1 : 25 entities Detail

Interaction partners 1. psalmotoxin1, : 1 interactors Detail