BMRBj - BMREntryMaster

Found 1 Document (1.953 seconds)

BMRB: 5513

2K3F

NMR assignment and secondary structure identification of the ribosomal protein L11 from Thermotoga maritima

Authors

Ilin, S., Hoskins, A., Schwalbe, H., Wohnert, J.

Assembly

Thermotoga maritima Ribosomal protein L11

Entity

1. Thermotoga maritima Ribosomal protein L11 (polymer, Thiol state: all free), 141 monomers, 15088.73 Da Detail

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

Formula weight

15088.73 Da

Source organism

Thermotoga maritima

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 95.3 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.3 % (1567 of 1644)	95.2 % (820 of 861)	94.7 % (610 of 644)	98.6 % (137 of 139)
Backbone	96.2 % (795 of 826)	96.8 % (273 of 282)	95.2 % (393 of 413)	98.5 % (129 of 131)
Sidechain	94.5 % (897 of 949)	94.5 % (547 of 579)	94.5 % (342 of 362)	100.0 % (8 of 8)
Aromatic	83.9 % (52 of 62)	83.9 % (26 of 31)	83.9 % (26 of 31)
Methyl	96.6 % (170 of 176)	96.6 % (85 of 88)	96.6 % (85 of 88)

1. Ribosomal protein L11

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMSP	methyl protons	internal	direct	1.0
¹⁵N	TMSP	methyl protons	null	indirect	0.1013291
¹³C	TMSP	methyl protons	null	indirect	0.25145

Referencing offset: 1.94 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMSP	methyl protons	internal	direct	1.0
¹⁵N	TMSP	methyl protons	null	indirect	0.1013291
¹³C	TMSP	methyl protons	null	indirect	0.25145

Referencing offset: 1.94 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMSP	methyl protons	internal	direct	1.0
¹⁵N	TMSP	methyl protons	null	indirect	0.1013291
¹³C	TMSP	methyl protons	null	indirect	0.25145

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMSP	methyl protons	internal	direct	1.0
¹⁵N	TMSP	methyl protons	null	indirect	0.1013291
¹³C	TMSP	methyl protons	null	indirect	0.25145

Referencing offset: 1.99 ppm, Outliers: 4 Detail

Coupling constant

111 J values in 1 lists
Temperature 298 (±0.1) K, pH 6.1 (±0.2) Detail

Release date

2003-02-17

Citation 1

NMR assignment of the full-length ribosomal protein L11 from Thermotoga maritima
Ilin, S., Hoskins, A., Schwalbe, H., Wohnert, J.
J. Biomol. NMR (2003), 25, 163-164, PubMed 12652127 , Abstract

Show more 3 citaions

Citation 2

1H, 15N, and 13C assignments and secondary structure identification for full-length ribosomal protein L11 from Thermus thermophilus
Markus, M.A., Triantafillidou, D., Choli-Papadopoulou, T., Torchia, D.A.
J. Biomol. NMR (2001), 20, 293-294, PubMed 11519754 , Abstract

Citation 3

A detailed view of a ribosomal active site: the structure of the L11-RNA complex
Wimberly, B.T., Guymon, R., McCutcheon, J.P., White, S.W., Ramakrishnan, V.
Cell (1999), 97, 491-502, PubMed 10338213 , Abstract

Citation 4

The RNA binding domain of ribosomal protein L11: three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA
Hinck, A.P., Markus, M.A., Huang, S., Grzesiek, S., Kustonovich, I., Draper, D.E., Torchia, D.A.
J. Mol. Biol. (1997), 274, 101-113, PubMed 9398519 , DOI 10.1006/jmbi.1997.1379 , Abstract

Hide non-primary 3 citaions

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 4965 released on 2001-03-31
    Title 1H, 15N, and 13C Assignments and Secondary Structure Identification for Full-length Ribosomal Protein L11 from Thermus thermophilus
  Swiss-Prot: P29395 released on 1992-12-01
    Title RL11_THEMA Entity 50S ribosomal protein L11

Related entities 1. Thermotoga maritima Ribosomal protein L11, : 1 : 9 : 10 : 287 entities Detail

Interaction partners 1. Thermotoga maritima Ribosomal protein L11, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

2 1H-13C HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

3 1H-13C CT-HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

4 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

5 HNCACB

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

6 HNCO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

7 HCC(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

8 CC(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

9 HBHA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

10 HCCH-TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

11 HCCH-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

#3 Model Varian UnityInova (600 MHz)

#4 Model Varian UnityInova (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	Ribosomal protein L11	[U-95% 13C; U-95% 15N]	1.3 mM
2	potassium phosphate		25 mM
3	potassium chloride		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
4	Ribosomal protein L11	[U-15N]	1.5 mM
5	potassium phosphate		25 mM
6	potassium chloride		50 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5513_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5513_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1731		97.2 (chain: 1, length: 141)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 141, Sequence coverage: 97.2%
- Total number of assignments
  - ¹H chemical shifts: 990
  - ¹³C chemical shifts: 604
  - ¹⁵N chemical shifts: 137
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	861	810	94.1
¹³C chemical shifts	644	604	93.8
¹⁵N chemical shifts	142	137	96.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	579	538	92.9
¹³C chemical shifts	362	338	93.4
¹⁵N chemical shifts	11	8	72.7

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Found 1 Document (1.953 seconds)

BMRB: 5513

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Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. Thermotoga maritima Ribosomal protein L11, : 1 : 9 : 10 : 287 entities Detail

Interaction partners 1. Thermotoga maritima Ribosomal protein L11, : 1 interactors Detail

Experiments performed 11 experiments Detail

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Chemical shift validation 3 contents Detail