BMRB: 5517

NMR studies of the DNA-binding domain of B-Myb

Authors

Jones, G.

Assembly

B-MybR2R3

Entity

1. B-MybR2R3-tom1a (polymer, Thiol state: all free), 110 monomers, 12930.69 Da Detail

GIPDLVKGPW TKEEDQKVIE LVKKYGTKQW TLIAKHLKGR LGKQCRERWH NHLNPEVKKS SWTEEEDRII FEAHKVLGNR WAEIAKLLPG RTDNAVKNHW NSTIKRKVDT

2. tom1a (polymer, Thiol state: not present), 16 monomers, 4961.163 Da Detail

CTCAGTCCGT TAAGGA

3. tom1a (polymer, Thiol state: not present), 16 monomers, 4961.163 Da Detail

TCCTTAACGG ACTGAG

Total weight

22853.016 Da

Max. entity weight

12930.69 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 72.5 %, Completeness: 57.1 %, Completeness (bb): 58.2 % Detail

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Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	1H	13C	15N
All	57.1 % (961 of 1682)	53.1 % (551 of 1037)	58.2 % (305 of 524)	86.8 % (105 of 121)
Backbone	58.2 % (510 of 876)	46.8 % (209 of 447)	61.3 % (198 of 323)	97.2 % (103 of 106)
Sidechain	58.9 % (535 of 909)	58.0 % (342 of 590)	62.8 % (191 of 304)	13.3 % (2 of 15)
Aromatic	0.0 % (0 of 174)	0.0 % (0 of 119)	0.0 % (0 of 49)	0.0 % (0 of 6)
Methyl	67.7 % (84 of 124)	68.2 % (45 of 66)	67.2 % (39 of 58)

1. B-MybR2R3-tom1a

GIPDLVKGPW TKEEDQKVIE LVKKYGTKQW TLIAKHLKGR LGKQCRERWH NHLNPEVKKS SWTEEEDRII FEAHKVLGNR WAEIAKLLPG RTDNAVKNHW NSTIKRKVDT

2. tom1a

CTCAGTCCGT TAAGGA

3. tom1a

TCCTTAACGG ACTGAG

Show sample condition

Sample

Temperature 293 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	B-MybR2R3-tom1a	[U-15N; U-13C]-All but Pro		1.9 mM
2	tom1a			1.9 mM
3	tom1a			1.9 mM

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LACS Plot; CA

Referencing offset: -0.55 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.55 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 32 models in PDB: 1A5J, Strand ID: A Detail

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Release date

2003-06-11

Citation 1

Sequence-specific assignment of the B-Myb DNA-binding domain (B-MybR2R3) bound to a 16 base-pair DNA target site corresponding to a regulatory site from the tom-1 gene
Jones, G.J., Howard, M., McIntosh, P., Williamson, R.A., Carr, M.D.
J. Biomol. NMR (2003), 26, 375-376, PubMed 12815265 , Abstract

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Citation 2

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

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Related entities 1. B-MybR2R3-tom1a, : 1 : 1 : 261 entities Detail

Experiments performed 6 experiments Detail