Role of backbone dynamics and structure in controlling the hydrolysis constants of serine proteinase inhibitors
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS8:SG | 2:CYS20:SG |
2 | disulfide | sing | 1:CYS16:SG | 2:CYS17:SG |
3 | disulfide | sing | 2:CYS6:SG | 2:CYS38:SG |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 10.0 % (37 of 369) | 5.8 % (18 of 311) | 32.8 % (19 of 58) |
Backbone | 22.3 % (37 of 166) | 15.9 % (18 of 113) | 35.8 % (19 of 53) |
Sidechain | 0.0 % (0 of 203) | 0.0 % (0 of 198) | 0.0 % (0 of 5) |
Aromatic | 0.0 % (0 of 22) | 0.0 % (0 of 22) | |
Methyl | 0.0 % (0 of 26) | 0.0 % (0 of 26) |
1. Ovomucoid Third Domain from Indian Peafowl
LAAVSVDCSE YPKPACTL2. Ovomucoid Third Domain from Indian Peafowl
EHRPLCGSDN KTYGNKCNFC NAVVESNGTL TLSHFGKCTemperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Ovomucoid Third Domain from Indian Peafowl | [U-13C; U-15N] | 2 mM | |
2 | Ovomucoid Third Domain from Indian Peafowl | [U-13C; U-15N] | 2 mM |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 14.6 % (108 of 738) | 8.7 % (54 of 622) | 46.6 % (54 of 116) |
Backbone | 32.5 % (108 of 332) | 23.9 % (54 of 226) | 50.9 % (54 of 106) |
Sidechain | 0.0 % (0 of 406) | 0.0 % (0 of 396) | 0.0 % (0 of 10) |
Aromatic | 0.0 % (0 of 44) | 0.0 % (0 of 44) | |
Methyl | 0.0 % (0 of 52) | 0.0 % (0 of 52) |
1. Ovomucoid Third Domain from Indian Peafowl
LAAVSVDCSE YPKPACTL2. Ovomucoid Third Domain from Indian Peafowl
EHRPLCGSDN KTYGNKCNFC NAVVESNGTL TLSHFGKCTemperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Ovomucoid Third Domain from Indian Peafowl | [U-13C; U-15N] | 2 mM | |
2 | Ovomucoid Third Domain from Indian Peafowl | [U-13C; U-15N] | 2 mM |