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BMRB: 5537

1UZC

Structure of an FF domain Human HYPA/FBP11

Authors

Assembly

Human FF domain

Entity

1. Human FF domain (polymer, Thiol state: not present), 71 monomers, 8236.344 Da Detail

GSQPAKKTYT WNTKEEAKQA FKELLKEKRV PSNASWEQAM KMIINDPRYS ALAKLSEKKQ AFNAYKVQTE K

Formula weight

8236.344 Da

Source organism

Exptl. method

Refine. method

DISTANCE GEOMETRY SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.1 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.1 % (826 of 887)	95.8 % (453 of 473)	88.4 % (296 of 335)	97.5 % (77 of 79)
Backbone	95.5 % (401 of 420)	95.0 % (133 of 140)	95.3 % (202 of 212)	97.1 % (66 of 68)
Sidechain	92.0 % (494 of 537)	96.1 % (320 of 333)	84.5 % (163 of 193)	100.0 % (11 of 11)
Aromatic	60.3 % (41 of 68)	100.0 % (34 of 34)	15.6 % (5 of 32)	100.0 % (2 of 2)
Methyl	94.8 % (55 of 58)	89.7 % (26 of 29)	100.0 % (29 of 29)

1. Human FF domain

GSQPAKKTYT WNTKEEAKQA FKELLKEKRV PSNASWEQAM KMIINDPRYS ALAKLSEKKQ AFNAYKVQTE K

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.1 ppm, Outliers: 3 Detail

LACS Plot; CO

Referencing offset: -0.3 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 23 models in PDB: 1UZC, Strand ID: A Detail

Release date

2003-02-20

Citation

The structure of an FF domain from human HYPA/FBP11
Allen, M.D., Friedler, A., Schon, O., Bycroft, M.
J. Mol. Biol. (2002), 323, 411-416, PubMed 12381297 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: O75400 released on 2003-08-22
    Title PR40A_HUMAN Entity Pre-mRNA-processing factor 40 homolog A
  Swiss-Prot: P24928 released on 1992-03-01
    Title RPB1_HUMAN Entity DNA-directed RNA polymerase II subunit RPB1

Related entities 1. Human FF domain, : 1 : 1 : 6 : 24 entities Detail

Interaction partners 1. Human FF domain, : 96 interactors Detail

More details for 96 interactors identified by 20 citations

Experiments performed 12 experiments Detail

1 1H NOESY

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

2 1H TOCSY

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

3 1H DQF-COSY

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

4 1H-15N NOESY

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

5 1H-15N TOCSY

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

6 HNCACB

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

7 CBCACONH

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

8 15N-HSQC

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

9 HN(CO)CA

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

10 HNCO

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

11 13C-HSQC

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

12 HNHB

Instrument

Bruker Advance - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human FF domain	[U-99% 13C; U-99% 15N]		2.0 mM
2	potassium phosphate			50 mM
3	sodium chloride			150 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5537_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5537_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	904		100.0 (chain: 1, length: 71)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 71, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 532
  - ¹³C chemical shifts: 295
  - ¹⁵N chemical shifts: 77
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords NMR structure, transcription, phosphopeptide recognition, RNA polymerase II carboxyl-terminal domain