BMRB: 5555

Solution structure of the 2nd PHD domain from Mi2b

Authors

Kwan, A.H.Y., Gell, D.A., Mackay, J.P.

Assembly

Chromodomain helicase-DNA-binding protein 4

Entity

1. PHD (polymer, Thiol state: not reported), 61 monomers, 6680.715 Da Detail

GPLGSDHHME FCRVCKDGGE LLCCDTCPSS YHIHCLNPPL PEIPNGEWLC PRCTCPALKG K

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

6811.5327 Da

Max. entity weight

6680.715 Da

Entity Connection

metal coordination 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:HIS32:ND1	2:ZN1:ZN
2	metal coordination	sing	1:CYS12:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS15:SG	2:ZN1:ZN
4	metal coordination	sing	1:CYS35:SG	2:ZN1:ZN
5	metal coordination	sing	1:CYS24:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS27:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS50:SG	2:ZN1:ZN
8	metal coordination	sing	1:CYS53:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.8 %, Completeness (bb): 96.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	90.8 % (373 of 411)	90.4 % (321 of 355)	92.9 % (52 of 56)
Backbone	96.0 % (166 of 173)	97.5 % (117 of 120)	92.5 % (49 of 53)
Sidechain	87.0 % (207 of 238)	86.8 % (204 of 235)	100.0 % (3 of 3)
Aromatic	75.0 % (18 of 24)	73.9 % (17 of 23)	100.0 % (1 of 1)
Methyl	100.0 % (23 of 23)	100.0 % (23 of 23)

1. PHD

GPLGSDHHME FCRVCKDGGE LLCCDTCPSS YHIHCLNPPL PEIPNGEWLC PRCTCPALKG K

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PHD			1 mM
2	DTT			1 mM
3	sodium phosphate buffer			10 mM
4	NaCl			150 mM
5	H2O			95 %
6	D2O			5 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1MM2, Strand ID: A Detail

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Release date

2003-07-31

Citation

Engineering a protein scaffold from a PHD finger
Kwan, A.H.Y., Gell, D.A., Verger, A., Crossley, M., Matthews, J.M., Mackay, J.P.
Structure (2003), 11, 803-813, PubMed 12842043 , DOI: , Abstract

Related entities 1. PHD, : 1 : 1 : 9 : 139 entities Detail

Interaction partners 1. PHD, : 69 interactors Detail

Experiments performed 4 experiments Detail