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BMRB: 5595

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Letter to the Editor: 1H, 13C, 15N backbone resonance assignments of apo-NosL, a novel 18.5 kDa copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes

Authors

Taubner, L.M., McGuirl, M.A., Dooley, D.M., Copie, V.

Assembly

NosL

Entity

1. NosL (polymer, Thiol state: all free), 175 monomers, 18537.62 Da Detail

MDKEDVAQSI VPQDMTPETL GHYCQMNLLE HPGPKAQIFL EGSPAPLFFS QVRDAIAYAR GPEQIAPILV IYVNDMGAAG ATWDQPGDGN WIAADKAFYV VGSARRGGMG APEAVPFSSR DEAAAFVLAE GGQVLALADI TDAMVLTPVE TGSEPRADDE DYLGRLRALP HPAGG

Formula weight

18537.62 Da

Source organism

Achromobacter cycloclastes

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 77.0 %, Completeness (bb): 91.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.0 % (1466 of 1905)	72.8 % (713 of 980)	80.2 % (603 of 752)	86.7 % (150 of 173)
Backbone	91.4 % (932 of 1020)	91.8 % (324 of 353)	90.3 % (458 of 507)	93.8 % (150 of 160)
Sidechain	65.5 % (683 of 1042)	62.0 % (389 of 627)	73.1 % (294 of 402)	0.0 % (0 of 13)
Aromatic	4.4 % (6 of 136)	5.9 % (4 of 68)	3.0 % (2 of 66)	0.0 % (0 of 2)
Methyl	81.1 % (159 of 196)	78.6 % (77 of 98)	83.7 % (82 of 98)

1. NosL

MDKEDVAQSI VPQDMTPETL GHYCQMNLLE HPGPKAQIFL EGSPAPLFFS QVRDAIAYAR GPEQIAPILV IYVNDMGAAG ATWDQPGDGN WIAADKAFYV VGSARRGGMG APEAVPFSSR DEAAAFVLAE GGQVLALADI TDAMVLTPVE TGSEPRADDE DYLGRLRALP HPAGG

Show sample condition

Sample

Temperature 303 (±1) K

#	Name	Isotope labeling	Concentration
1	NosL	[U-15N; U-13C]	1.0 mM
2	DTT	[U-2D]	1.0 mM
3	EDTA		1.0 mM
4	D2O		10 %
5	sodium phosphate		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.19 ppm, Outliers: 1 Detail

Release date

2004-03-14

Citation 1

1H, 13C, 15N backbone and sidechain resonance assignments of apo-NosL, a novel copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes
Taubner, L.M., McGuirl, M.A., Dooley, D.M., Copie, V.V.
J. Biomol. NMR (2004), 29, 211-212, PubMed 15014237 , DOI 10.1023/B:JNMR.0000019240.29152.ac , Abstract

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Citation 2

Expression, purification, and characterization of NosL, a novel Cu(I) protein of the nitrous oxide reductase (nos) gene cluster
McGuirl, M.A., Bollinger, J.A., Cosper, N., Scott, R.A., Dooley, D.M.
J. Biol. Inorg. Chem. (2001), 6, 189-195, PubMed 11293413 , DOI: , Abstract

Citation 3

The nos (nitrous oxide reductase) gene cluster from the soil bacterium Achromobacter cycloclastes: cloning, sequence analysis, and expression
McGuirl, M.A., Nelson, L.K., Bollinger, J.A., Chan, Y.K., Dooley, D.M.
J. Inorg. Biochem. (1998), 70, 155-169, PubMed 9720302 , DOI: , Abstract

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Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P94127 released on 1997-11-01
Title NOSZ_ACHCY Entity Nitrous-oxide reductase

Related entities 1. NosL, : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

1 15N HSQC