Letter to the Editor: 1H, 13C, 15N backbone resonance assignments of apo-NosL, a novel 18.5 kDa copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes
MDKEDVAQSI VPQDMTPETL GHYCQMNLLE HPGPKAQIFL EGSPAPLFFS QVRDAIAYAR GPEQIAPILV IYVNDMGAAG ATWDQPGDGN WIAADKAFYV VGSARRGGMG APEAVPFSSR DEAAAFVLAE GGQVLALADI TDAMVLTPVE TGSEPRADDE DYLGRLRALP HPAGG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.0 % (1466 of 1905) | 72.8 % (713 of 980) | 80.2 % (603 of 752) | 86.7 % (150 of 173) |
Backbone | 91.4 % (932 of 1020) | 91.8 % (324 of 353) | 90.3 % (458 of 507) | 93.8 % (150 of 160) |
Sidechain | 65.5 % (683 of 1042) | 62.0 % (389 of 627) | 73.1 % (294 of 402) | 0.0 % (0 of 13) |
Aromatic | 4.4 % (6 of 136) | 5.9 % (4 of 68) | 3.0 % (2 of 66) | 0.0 % (0 of 2) |
Methyl | 81.1 % (159 of 196) | 78.6 % (77 of 98) | 83.7 % (82 of 98) |
1. NosL
MDKEDVAQSI VPQDMTPETL GHYCQMNLLE HPGPKAQIFL EGSPAPLFFS QVRDAIAYAR GPEQIAPILV IYVNDMGAAG ATWDQPGDGN WIAADKAFYV VGSARRGGMG APEAVPFSSR DEAAAFVLAE GGQVLALADI TDAMVLTPVE TGSEPRADDE DYLGRLRALP HPAGGTemperature 303 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NosL | [U-15N; U-13C] | 1.0 mM | |
2 | DTT | [U-2D] | 1.0 mM | |
3 | EDTA | 1.0 mM | ||
4 | D2O | 10 % | ||
5 | sodium phosphate | 100 mM |