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BMRB: 5658

3SDL

Sequence specific backbone assignment of 13C, 2H, 15N labeled ISG15

Authors

Yin, C., Cort, J.R., Swapna, G.V.T., Krug, R.M., Kennedy, M.A., Montelione, G.T.

Assembly

ISG15

Entity

1. ISG15 (polymer, Thiol state: all free), 164 monomers, 18055.45 Da Detail

SHHHHHHMGW DLTVKMLAGN EFQVSLSSSM SVSELKAQIT QKIGVHAFQQ RLAVHPSGVA LQDRVPLASQ GLGPGSTVLL VVDKCDEPLS ILVRNNKGRS STYEVRLTQT VAHLKQQVSG LEGVQDDLFW LTFEGKPLED QLPLGEYGLK PLSTVFMNLR LRGG

Formula weight

18055.45 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 51.2 %, Completeness (bb): 78.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.2 % (971 of 1896)	28.1 % (276 of 982)	72.0 % (532 of 739)	93.1 % (163 of 175)
Backbone	78.4 % (760 of 970)	47.0 % (158 of 336)	95.0 % (453 of 477)	94.9 % (149 of 157)
Sidechain	32.8 % (353 of 1075)	18.3 % (118 of 646)	53.8 % (221 of 411)	77.8 % (14 of 18)
Aromatic	3.2 % (4 of 126)	3.2 % (2 of 63)	0.0 % (0 of 61)	100.0 % (2 of 2)
Methyl	79.7 % (161 of 202)	77.2 % (78 of 101)	82.2 % (83 of 101)

1. ISG15

SHHHHHHMGW DLTVKMLAGN EFQVSLSSSM SVSELKAQIT QKIGVHAFQQ RLAVHPSGVA LQDRVPLASQ GLGPGSTVLL VVDKCDEPLS ILVRNNKGRS STYEVRLTQT VAHLKQQVSG LEGVQDDLFW LTFEGKPLED QLPLGEYGLK PLSTVFMNLR LRGG

Show sample condition

Sample

Temperature 291 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Interferon-Stimulated gene 15 product	[U-70% 2H; U-100% 13C; U-100% 15N]	0.7 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	DTT		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.86 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.86 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.48 ppm, Outliers: 1 Detail

Release date

2003-02-24

Citation 1

Backbone and Ile-δ1, Leu, Val methyl 1H, 13C and 15N NMR chemical shift assignments for human interferon-stimulated gene 15 protein
Yin, C., Aramini, J.M., Ma, L., Cort, J.R., Swapna, G.V.T., Krug, R.M., Montelione, G.T.
Biomol. NMR Assign. (2011), 5, 215-219, PubMed 21544738 , DOI 10.1007/s12104-011-9303-8 , Abstract

Show more 2 citaions

Citation 2

Automated analysis of protein NMR assignments using methods from artificial intelligence
Zimmerman, D.E., Kulikowski, C.A., Huang, Y., Feng, W., Tashiro, M., Shimotakahara, S., Chien, C., Powers, R., Montelione, G.T.
J. Mol. Biol. (1997), 269, 592-610, PubMed 9217263 , DOI 10.1006/jmbi.1997.1052 , Abstract

Citation 3

Automatic determination of protein backbone resonance assignments from triple resonance nuclear magnetic resonance data
Moseley, H.N., Monleon, D., Montelione, G.T.
Methods. Enzymol. (2001), 339, 91-108, PubMed 11462827 , DOI: , Abstract

Hide non-primary 2 citaions

Related entities 1. ISG15, : 1 : 11 : 489 entities Detail

Interaction partners 1. ISG15, : 14 interactors Detail

More details for 14 interactors identified by 11 citations

Experiments performed 8 experiments Detail

1 1H-15N NOESY