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BMRB: 5691

1NY4

Solution Structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19

Authors

Aramini, J.M., Cort, J.R., Huang, Y.J., Xiao, R., Acton, T.B., Ho, C.K., Shih, L., Kennedy, M.A., Montelione, G.T.

Assembly

30S ribosomal protein S28E

Entity

1. 30S ribosomal protein S28E (polymer, Thiol state: not present), 82 monomers, 9364.512 Da Detail

MAEDEGYPAE VIEIIGRTGT TGDVTQVKVR ILEGRDKGRV IRRNVRGPVR VGDILILRET EREAREIKSR RAAALEHHHH HH

Formula weight

9364.512 Da

Source organism

Pyrococcus horikoshii

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 89.2 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.2 % (836 of 937)	87.8 % (432 of 492)	90.4 % (328 of 363)	92.7 % (76 of 82)
Backbone	92.0 % (449 of 488)	91.8 % (156 of 170)	92.0 % (219 of 238)	92.5 % (74 of 80)
Sidechain	87.0 % (455 of 523)	85.7 % (276 of 322)	88.9 % (177 of 199)	100.0 % (2 of 2)
Aromatic	25.0 % (8 of 32)	25.0 % (4 of 16)	25.0 % (4 of 16)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. RS28 PYRHO

MAEDEGYPAE VIEIIGRTGT TGDVTQVKVR ILEGRDKGRV IRRNVRGPVR VGDILILRET EREAREIKSR RAAALEHHHH HH

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.18 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.18 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1NY4, Strand ID: A Detail

Coupling constant

45 J values in 1 lists
Temperature 293 (±0.5) K, pH 6.5 (±0.1) Detail

Release date

2003-02-24

Citation 1

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii
Kennedy, M.A., Acton, T.B., Montelione, G.T., Aramini, J.M., Huang, Y.J., Cort, J.R., Goldsmith-Fischman, S., Xiao, R., Shih, L., Ho, C.K., Liu, J., Rost, B., Honig, B.
Protein Sci. (2003), 12, 2823-2830, PubMed 14627742 , DOI 10.1110/ps.03359003 , Abstract

Show more 5 citaions

Citation 2

Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3
Sakai, M., Ogura, K., Otsuka, R., Nakazawa, H., Takamiya, M., Ohfuku, Y., Funahashi, T., Tanaka, T., Kudoh, Y., Yamazaki, J., Aoki, K., Oguchi, A., Kushida, N., Kikuchi, H., Kawarabayasi, Y., Sawada, M., Horikawa, H., Haikawa, Y., Hino, Y., Yamamoto, S., Sekine, M., Baba, S., Kosugi, H., Hosoyama, A., Nagai, Y.
DNA Res. (1998), 5, 55-76, PubMed 9679194 , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Citation 4

Automated analysis of protein NMR assignments using methods from artificial intelligence
Zimmerman, D.E., Kulikowski, C.A., Huang, Y., Feng, W., Tashiro, M., Shimotakahara, S., Chien, C., Powers, R., Montelione, G.T.
J. Mol. Biol. (1997), 269, 592-610, PubMed 9217263 , DOI 10.1006/jmbi.1997.1052 , Abstract

Citation 5

Protein backbone angle restraints from searching a database for chemical shift and sequence homology
Cornilescu, G., Delaglio, F., Bax, A.
J. Biomol. NMR (1999), 13, 289-302, PubMed 10212987 , Abstract

Citation 6

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Hide non-primary 5 citaions

Related entities 1. 30S ribosomal protein S28E, : 1 : 7 : 102 entities Detail

Experiments performed 24 experiments Detail

1 1H,15N-HSQC (regular)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

2 1H,15N-HSQC (NH2 only)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

3 1H,15N-HSQC (full SW)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

4 1H,13C-HSQC (aliph)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

5 1H,13C-HSQC (arom)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

6 3D 1H-15N NOESY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

7 3D 1H-13C NOESY (aliph)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

8 3D 1H-13C NOESY (arom)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

9 4D 13C NOESY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

10 HNCO

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

11 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

12 HNCACB

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

13 CBCACO(CA)HA

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

14 HA(CA)NH

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

15 HA(CACO)NH

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

16 (H)CC(CO)NH TOCSY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

17 H(CCCO)NH TOCSY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

18 HcCH COSY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

19 HcCH TOCSY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

20 HNHA

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

21 1H,13C-HSQC (high res)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

22 H/D exchange (6 min)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

23 H/D exchange (60 min)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

24 H/D exchange (8 hrs)

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (750 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian INOVA (600 MHz)

#5 Model Varian INOVA (500 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %
7	D2O		5 %

Sample #2

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
8	RS28_PYRHO	[U-100% 13C; U-100% 15N]	1.0 mM
9	MES		20 mM
10	NaCl		100 mM
11	CaCl2		5 mM
12	DTT		10 mM
13	NaN3		0.02 %
14	D2O		100 %

Sample #3

Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
15	RS28_PYRHO	[U-5% 13C; U-100% 15N]	1.0 mM
16	MES		20 mM
17	NaCl		100 mM
18	CaCl2		5 mM
19	DTT		10 mM
20	NaN3		0.02 %
21	D2O		5 %

nullKeywords structural genomics, Northeast Structural Genomics Consortium, JR19, ribosomal protein

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BMRB: 5691

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Related entities 1. 30S ribosomal protein S28E, : 1 : 7 : 102 entities Detail

Experiments performed 24 experiments Detail

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