BMRBj - BMREntryMaster

Found 1 Document (1.145 seconds)

BMRB: 5703

1NZ1

SP phosphorothioate U6 RNA ISL

Authors

Reiter, N.J., Butcher, S.E.

Assembly

U6 snRNA intramolecular stem loop

Entity

1. U6 snRNA intramolecular stem loop (polymer, Thiol state: not present), 24 monomers, 7747.651 Da Detail

GGUUCCCCUG CAUAAGGAXG AACC

Formula weight

7747.651 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 35.4 %, Completeness (bb): 16.7 % Detail

Polymer type: polyribonucleotide

	Total	¹³C	³¹P
All	35.4 % (63 of 178)	40.6 % (63 of 155)	0.0 % (0 of 23)
Suger, PO4	16.7 % (23 of 138)	20.0 % (23 of 115)	0.0 % (0 of 23)
Nucleobase	100.0 % (40 of 40)	100.0 % (40 of 40)
Aromatic	100.0 % (40 of 40)	100.0 % (40 of 40)

1. U6 snRNA

GGUUCCCCUG CAUAAGGAXG AACC

Show sample condition

Sample

Temperature 283 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
1	U6 snRNA	1 mM
2	NaCl	50 mM
3	H2O	90 %
4	D2O	10 %

Release date

2003-08-06

Citation

Structure of the U6 RNA intramolecular stem-loop harboring an S(P)-phosphorothioate modification
Reiter, N.J., Nikstad, L.J., Allmann, A.M., Johnson, R.J., Butcher, S.E.
RNA (2003), 9, 533-542, PubMed 12702812 , DOI: , Abstract

Related entities 1. U6 snRNA intramolecular stem loop, : 1 : 10 entities Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5703_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
1	19	SSU	URIDINE-5'-PHOSPHOROTHIOATE	Assigned chemical shifts

Content subtype: bmr5703_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	271		100.0 (chain: 1, length: 24)
1	Chemical shift references	chemical_shift_reference	OK	4		No information

Assigned chemical shifts

Saveframe: shift_set_1

Status: Warning
Sequence coverage of experimental data
- Entity_assembly_ID: 1, Chain length: 24, Sequence coverage: 100.0%
Total number of assignments
- ¹H chemical shifts: 204
- ¹³C chemical shifts: 66
- ³¹P chemical shifts: 1

Completeness of assignments

Entity_assemble_ID: 1

Excluded Comp_ID in statistics

Comp_index_ID	Comp_ID
19	SSU

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
2	G	H21	8.59
2	G	H22	6.19
10	G	H21	8.16
10	G	H22	7.13
12	A	H61	7.91
12	A	H62	6.63
14	A	H61	8.44
14	A	H62	6.84
15	A	H61	7.76
15	A	H62	6.63
16	G	H21	8.2
16	G	H22	5.94
17	G	H21	8.44
17	G	H22	6.7
20	G	H21	7.913
20	G	H22	5.927
21	A	H61	8.06
21	A	H62	6.58
22	A	H61	8.31
22	A	H62	6.73

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	202	178	88.1
¹³C chemical shifts	155	63	40.6

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	138	120	87.0
¹³C chemical shifts	115	23	20.0

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	58	90.6
¹³C chemical shifts	40	40	100.0

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	18	100.0
¹³C chemical shifts	18	18	100.0

Histogram of normalized assigned chemical shifts

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Found 1 Document (1.145 seconds)

BMRB: 5703

Sample

Related entities 1. U6 snRNA intramolecular stem loop, : 1 : 10 entities Detail

Chemical shift validation 3 contents Detail