Influence of pH on NMR structure and stability of the human prion protein globular domain
GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY ERESQAYYQR GS
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS61:SG | 1:CYS96:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.5 % (1144 of 1308) | 97.2 % (663 of 682) | 72.4 % (364 of 503) | 95.1 % (117 of 123) |
Backbone | 82.0 % (546 of 666) | 97.0 % (223 of 230) | 67.3 % (220 of 327) | 94.5 % (103 of 109) |
Sidechain | 93.7 % (698 of 745) | 97.3 % (440 of 452) | 87.5 % (244 of 279) | 100.0 % (14 of 14) |
Aromatic | 71.6 % (96 of 134) | 91.0 % (61 of 67) | 52.2 % (35 of 67) | |
Methyl | 100.0 % (86 of 86) | 100.0 % (43 of 43) | 100.0 % (43 of 43) |
1. human prion protein
GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY ERESQAYYQR GSTemperature 298 (±1) K, pH 7.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prion protein | 0.8 ~ 1.3 mM |