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Found 1 Document (3.833 seconds)

BMRB: 5754

1EGX

1H, 13C and 15N backbone resonance assignment of the VASP EVH1 domain

Authors

Vogtherr, M., Grimme, S., Pescatore, B., Schwalbe, H.

Assembly

VASP EVH1 domain

Entity

1. VASP EVH1 domain (polymer, Thiol state: all free), 115 monomers, 12732.24 Da Detail

MSETVICSSR ATVMLYDDGN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV GRKMQPDQQV VINCAIVRGV KYNQATPNFH QWRDARQVWG LNFGSKEDAA QFAAGMASAL EALEG

Formula weight

12732.24 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 64.2 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.2 % (843 of 1314)	58.1 % (396 of 682)	67.8 % (341 of 503)	82.2 % (106 of 129)
Backbone	95.0 % (646 of 680)	91.0 % (213 of 234)	97.6 % (328 of 336)	95.5 % (105 of 110)
Sidechain	40.5 % (300 of 740)	40.8 % (183 of 448)	42.5 % (116 of 273)	5.3 % (1 of 19)
Aromatic	0.0 % (0 of 118)	0.0 % (0 of 59)	0.0 % (0 of 56)	0.0 % (0 of 3)
Methyl	48.2 % (55 of 114)	61.4 % (35 of 57)	35.1 % (20 of 57)

1. Human VASP Ena-Vasp homology domain 1

MSETVICSSR ATVMLYDDGN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV GRKMQPDQQV VINCAIVRGV KYNQATPNFH QWRDARQVWG LNFGSKEDAA QFAAGMASAL EALEG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Human VASP Ena-Vasp homology domain 1	[U-13C; U-15N]	1.0 mM
2	DTT		10 mM
3	KCl		50 mM
4	KPi		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.37 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.37 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.62 ppm, Outliers: 1 Detail

Release date

2004-04-06

Citation 1

1H, 13C and 15N backbone resonance assignment of the VASP EVH1 domain
Vogtherr, M., Grimme, S., Pescatore, B., Schwalbe, H.
J. Biomol. NMR (2003), 27, 189-190, PubMed 12913419 , Abstract

Show more 2 citaions

Citation 2

Protein backbone angle restraints from searching a database for chemical shift and sequence homology
Cornilescu, G., Delaglio, F., Bax, A.
J. Biomol. NMR (1999), 13, 289-302, PubMed 10212987 , Abstract

Citation 3

Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity
Ball, L.J., Koehne, R., Hoffmann, B., Hoefner, A., Schmieder, P., Volkmer-Engert, R., Hof, M., Wahl, M., Schneider-Mergener, J., Walter, U., Oschkinat, H., Jarchau, T.
EMBO J. (2000), 19, 4903-4914, PubMed 10990454 , DOI 10.1093/emboj/19.18.4903 , Abstract

Hide non-primary 2 citaions

Related entities 1. VASP EVH1 domain, : 1 : 2 : 167 entities Detail

Interaction partners 1. VASP EVH1 domain, : 41 interactors Detail

More details for 41 interactors identified by 22 citations

Experiments performed 5 experiments Detail

#	Name	Isotope labeling	Concentration
5	Human VASP Ena-Vasp homology domain 1	[U-15N]	1.0 mM
6	DTT		10 mM
7	KCl		50 mM
8	KPi		20 mM

#	Name	Isotope labeling	Concentration
5	Human VASP Ena-Vasp homology domain 1	[U-15N]	1.0 mM
6	DTT		10 mM
7	KCl		50 mM
8	KPi		20 mM

#	Name	Isotope labeling	Concentration
5	Human VASP Ena-Vasp homology domain 1	[U-15N]	1.0 mM
6	DTT		10 mM
7	KCl		50 mM
8	KPi		20 mM

#	Name	Isotope labeling	Concentration
5	Human VASP Ena-Vasp homology domain 1	[U-15N]	1.0 mM
6	DTT		10 mM
7	KCl		50 mM
8	KPi		20 mM

#	Name	Isotope labeling	Concentration
5	Human VASP Ena-Vasp homology domain 1	[U-15N]	1.0 mM
6	DTT		10 mM
7	KCl		50 mM
8	KPi		20 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5754_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5754_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	902		100.0 (chain: 1, length: 115)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 100.0%
- Total number of assignments
  - ¹⁵N chemical shifts: 110
  - ¹H chemical shifts: 460
  - ¹³C chemical shifts: 332
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	137	110	80.3
¹H chemical shifts	682	390	57.2
¹³C chemical shifts	503	332	66.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	110	110	100.0
¹H chemical shifts	234	220	94.0
¹³C chemical shifts	230	226	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	27	0	0.0
¹H chemical shifts	448	170	37.9
¹³C chemical shifts	273	106	38.8

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Found 1 Document (3.833 seconds)

BMRB: 5754

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. VASP EVH1 domain, : 1 : 2 : 167 entities Detail

Interaction partners 1. VASP EVH1 domain, : 41 interactors Detail

Experiments performed 5 experiments Detail

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Chemical shift validation 3 contents Detail