A model for effector activity in a highly specific biological electron transfer complex: The cytochrome P450cam-putidaredoxin couple
TTETIQSNAN LAPLPPHVPE HLVFDFDMYN PSNLSAGVQE AWAVLQESNV PDLVWTRCNG GHWIATRGQL IREAYEDYRH FSSECPFIPR EAGEAYDFIP TSMDPPEQRQ FRALANQVVG MPVVDKLENR IQELACSLIE SLRPQGQCNF TEDYAEPFPI RIFMLLAGLP EEDIPHLKYL TDQMTRPDGS MTFAEAKEAL YDYLIPIIEQ RRQKPGTDAI SIVANGQVNG RPITSDEAKR MCGLLLVGGL DTVVNFLSFS MEFLAKSPEH RQELIERPER IPAACEELLR RFSLVADGRI LTSDYEFHGV QLKKGDQILL PQMLSGLDER ENAAPMHVDF SRQKVSHTTF GHGSHLCLGQ HLARREIIVT LKEWLTRIPD FSIAPGAQIQ HKSGIVSGVQ ALPLVWDPAT TKAV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 21.2 % (1022 of 4830) | 13.2 % (333 of 2515) | 27.5 % (520 of 1890) | 39.8 % (169 of 425) |
Backbone | 35.1 % (851 of 2424) | 24.7 % (203 of 823) | 39.4 % (480 of 1217) | 43.8 % (168 of 384) |
Sidechain | 11.1 % (309 of 2795) | 7.7 % (131 of 1692) | 16.7 % (177 of 1062) | 2.4 % (1 of 41) |
Aromatic | 2.2 % (8 of 364) | 2.2 % (4 of 182) | 2.3 % (4 of 177) | 0.0 % (0 of 5) |
Methyl | 10.6 % (50 of 472) | 9.3 % (22 of 236) | 11.9 % (28 of 236) |
1. cytochrome P450cam
TTETIQSNAN LAPLPPHVPE HLVFDFDMYN PSNLSAGVQE AWAVLQESNV PDLVWTRCNG GHWIATRGQL IREAYEDYRH FSSECPFIPR EAGEAYDFIP TSMDPPEQRQ FRALANQVVG MPVVDKLENR IQELACSLIE SLRPQGQCNF TEDYAEPFPI RIFMLLAGLP EEDIPHLKYL TDQMTRPDGS MTFAEAKEAL YDYLIPIIEQ RRQKPGTDAI SIVANGQVNG RPITSDEAKR MCGLLLVGGL DTVVNFLSFS MEFLAKSPEH RQELIERPER IPAACEELLR RFSLVADGRI LTSDYEFHGV QLKKGDQILL PQMLSGLDER ENAAPMHVDF SRQKVSHTTF GHGSHLCLGQ HLARREIIVT LKEWLTRIPD FSIAPGAQIQ HKSGIVSGVQ ALPLVWDPAT TKAVTemperature 308 (±0.1) K, pH 7.4 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome P450cam | [U-15N; U-13C, U-85% 2H] | 0.2 ~ 0.6 mM | |
2 | TrisCl | [U-2H] | 50 mM | |
3 | KCl | 150 mM | ||
4 | camphor | [U-2H] | 1 mM | |
5 | H2O | 90 % | ||
6 | D2O | 10 % |