Solution Structure of the disulphide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS57:SG | 1:CYS57:SG |
2 | disulfide | sing | 1:CYS11:SG | 1:CYS37:SG |
3 | disulfide | sing | 1:CYS11:SG | 1:CYS37:SG |
4 | disulfide | sing | 1:CYS21:SG | 1:CYS36:SG |
5 | disulfide | sing | 1:CYS21:SG | 1:CYS36:SG |
6 | disulfide | sing | 1:CYS31:SG | 1:CYS48:SG |
7 | disulfide | sing | 1:CYS31:SG | 1:CYS48:SG |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 78.1 % (318 of 407) | 75.6 % (263 of 348) | 93.2 % (55 of 59) |
Backbone | 90.5 % (153 of 169) | 89.7 % (104 of 116) | 92.5 % (49 of 53) |
Sidechain | 69.3 % (165 of 238) | 68.5 % (159 of 232) | 100.0 % (6 of 6) |
Aromatic | 50.0 % (16 of 32) | 48.4 % (15 of 31) | 100.0 % (1 of 1) |
Methyl | 95.5 % (21 of 22) | 95.5 % (21 of 22) |
1. human intestinal trefoil factor 3
EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTFTemperature 308 (±0.1) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human intestinal trefoil factor 3 | [U-15N] | 1.0 ~ 4.0 mM |