BMRBj - BMREntryMaster

Found 1 Document (1.99 seconds)

BMRB: 5784

1PY4

1H assignment of a human variant of beta2-microglobulin where His 31 was replaced by a Tyr

Authors

Pettirossi, F., Corazza, A., Viglino, P., Verdone, G., Esposito, G.

Assembly

His31Tyrbeta2-microglobulin

Entity

1. His31Tyrbeta2-microglobulin (polymer, Thiol state: all disulfide bound), 100 monomers, 11888.25 Da Detail

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FYPSDIEVDL LKNGERIEKV EHSDLSFSKD WSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Formula weight

11888.25 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS26:SG	1:CYS81:SG

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 74.6 %, Completeness (bb): 88.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	74.6 % (478 of 641)	74.6 % (478 of 641)
Backbone	88.4 % (175 of 198)	88.4 % (175 of 198)
Sidechain	68.4 % (303 of 443)	68.4 % (303 of 443)
Aromatic	47.9 % (34 of 71)	47.9 % (34 of 71)
Methyl	91.1 % (41 of 45)	91.1 % (41 of 45)

1. beta2-microglobulin

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FYPSDIEVDL LKNGERIEKV EHSDLSFSKD WSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Show sample condition

Sample

Temperature 303 (±0.1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	beta2-microglobulin	0.8 mM
2	sodium phosphate	70 mM
3	sodium chloride	100 mM
4	H2O	100 %

Release date

2003-04-23

Citation 1

Properties of some variants of human beta2-microglobulin and amyloidogenesis
Corazza, A., Pettirossi, F., Viglino, P., Verdone, G., Garcia, J., Dumy, P., Giorgetti, S., Mangione, P., Andreola, A., Stoppini, M., Bellotti, V., Esposito, G.
J. Biol. Chem. (2004), 279, 9176-9189, PubMed 14660575 , DOI 10.1074/jbc.M310779200 , Abstract

Show more 2 citaions

Citation 2

The solution structure of human beta2-microglobulin reveals the prodromes of its amyloid transition
Verdone, G., Corazza, A., Viglino, P., Pettirossi, F., Giorgetti, S., Mangione, P., Andreola, A., Stoppini, M., Bellotti, V., Esposito, G.
Protein Sci. (2002), 11, 487-499, PubMed 11847272 , DOI 10.1110/ps.29002 , Abstract

Citation 3

Removal of the N-terminal hexapeptide from human beta2-microglobulin facilitates protein aggregation and fibril formation
Esposito, G., Michelutti, R., Verdone, G., Viglino, P., Hernandez, H., Robinson, C.V., Amoresano, A., Dal Piaz, F., Monti, M., Pucci, P., Mangione, P., Stoppini, M., Merlini, G., Ferri, G., Bellotti, V.
Protein Sci. (2000), 9, 831-845, PubMed 10850793 , DOI 10.1110/ps.9.5.831 , Abstract

Hide non-primary 2 citaions

Related entities 1. His31Tyrbeta2-microglobulin, : 1 : 99 : 188 entities Detail

Interaction partners 1. His31Tyrbeta2-microglobulin, : 121 interactors Detail

More details for 121 interactors identified by 43 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5784_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:26:CYS:SG	1:81:CYS:SG	unknown	unknown	n/a

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5784_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	541		88.0 (chain: 1, length: 100)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 100, Sequence coverage: 88.0%
- Total number of assignments
  - ¹H chemical shifts: 541
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
87	THR	HG1	7.653

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	641	460	71.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	198	171	86.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	443	289	65.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	40	85.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	34	47.9

Keywords amyloidosis, beta2-microglobulin

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Found 1 Document (1.99 seconds)

BMRB: 5784

Sample

Related entities 1. His31Tyrbeta2-microglobulin, : 1 : 99 : 188 entities Detail

Interaction partners 1. His31Tyrbeta2-microglobulin, : 121 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail