BMRB: 5804

Three-disulfide variant of hen lysozyme: C76A/C94A

Authors

Yokota, A., Hirai, K., Miyauchi, H., Noda, Y., Inoue, K., Akasaka, K., Tachibana, H., Segawa, S.

Assembly

hen egg white lysozyme

Entity

1. hen egg white lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14380.07 Da Detail

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLANIP CSALLSSDIT ASVNAAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Formula weight

14380.07 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS128:SG
2	disulfide	sing	1:CYS31:SG	1:CYS116:SG
3	disulfide	sing	1:CYS65:SG	1:CYS81:SG

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 87.6 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.6 % (663 of 757)	87.6 % (663 of 757)
Backbone	97.0 % (262 of 270)	97.0 % (262 of 270)
Sidechain	82.3 % (401 of 487)	82.3 % (401 of 487)
Aromatic	100.0 % (65 of 65)	100.0 % (65 of 65)
Methyl	96.7 % (59 of 61)	96.7 % (59 of 61)

1. three-disulfide variant of hen lysozyme

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLANIP CSALLSSDIT ASVNAAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 3.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	three-disulfide variant of hen lysozyme	[U-15N]		0.3 ~ 1.0 mM

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Release date

2004-09-13

Citation 1

NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins
Yokota, A., Hirai, K., Miyauchi, H., Iimura, S., Noda, Y., Inoue, K., Akasaka, K., Tachibana, H., Segawa, S.
Biochemistry (2004), 43, 6663-6669, PubMed 15157100 , DOI 10.1021/bi049967w , Abstract

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Citation 2

Characterisation of the dominant oxidative folding intermediate of hen lysozyme
van den Berg, B., Chung, E.W., Robinson, C.V., Dobson, C.M.
J. Mol. Biol. (1999), 290, 781-796, PubMed 10395829 , DOI 10.1006/jmbi.1999.2915 , Abstract

Citation 3

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , DOI 10.1021/bi0113418 , Abstract

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Related entities 1. hen egg white lysozyme, : 1 : 105 : 142 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

Experiments performed 7 experiments Detail

Chemical shift validation 3 contents Detail