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1H assignments for yeast nucleoporin Nsp1 residues 528-557
Authors
Morrison, J., Yang, J., Stewart, M., Neuhaus, D.
Assembly
nucleoporin Nsp1 528-557
Entity
1. nucleoporin Nsp1 528-557 (polymer, Thiol state: not present), 30 monomers, 3208.486 Da Detail

KPAFSFGAKA NEKKESDESK SAFSFGSKPT


Formula weight
3208.486 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.7 %, Completeness: 71.7 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

Total1H
All71.7 % (132 of 184)71.7 % (132 of 184)
Backbone95.0 % (57 of 60)95.0 % (57 of 60)
Sidechain60.5 % (75 of 124)60.5 % (75 of 124)
Aromatic100.0 % (20 of 20)100.0 % (20 of 20)
Methyl100.0 % (5 of 5)100.0 % (5 of 5)

1. nucleoporin Nsp1 528-557

KPAFSFGAKA NEKKESDESK SAFSFGSKPT

Sample

Temperature 300 (±0.5) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
1nucleoporin Nsp1 528-5571.0 ~ 2.0 mM

Release date
2003-12-18
Citation
Solution NMR study of the interaction between NTF2 and nucleoporin FxFG repeats
Morrison, J., Yang, J., Stewart, M., Neuhaus, D.
J. Mol. Biol. (2003), 333, 587-603, PubMed 14556747 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 5887 released on 2005-09-15
    Title 1H, 15N and 13C assignments for nuclear transport factor 2 (NTF2)
  BMRB: 5888 released on 2003-12-18
    Title Backbone 1H, 15N, CA and CB assignments for the W7A mutant of rat nuclear transport factor 2 (NTF2)
Related entities 1. nucleoporin Nsp1 528-557, : 2 : 2 : 33 entities Detail
Interaction partners 1. nucleoporin Nsp1 528-557, : 44 interactors Detail
Experiments performed 3 experiments Detail
Chemical shift validation 3 contents Detail