BMRB: 5908

Backbone 1HN and 15N Chemical Shift Assignments for Sac7d V30I

Authors

Clark, A.T., McCrary, B.S., Edmondson, S.P., Shriver, J.W.

Assembly

Sac7d V30I monomer

Entity

1. Sac7d V30I monomer (polymer, Thiol state: not present), 66 monomers, 7622.794 Da Detail

MVKVKFKYKG EEKEVDTSKI KKVWRVGKMI SFTYDDNGKT GRGAVSEKDA PKELLDMLAR AEREKK

Formula weight

7622.794 Da

Source organism

Sulfolobus acidocaldarius

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 31.7 %, Completeness (bb): 66.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	31.7 % (158 of 499)	21.3 % (92 of 432)	98.5 % (66 of 67)
Backbone	66.2 % (133 of 201)	50.7 % (69 of 136)	98.5 % (64 of 65)
Sidechain	8.4 % (25 of 298)	7.8 % (23 of 296)	100.0 % (2 of 2)
Aromatic	20.0 % (5 of 25)	16.7 % (4 of 24)	100.0 % (1 of 1)
Methyl	6.9 % (2 of 29)	6.9 % (2 of 29)

1. Sac7d

MVKVKFKYKG EEKEVDTSKI KKVWRVGKMI SFTYDDNGKT GRGAVSEKDA PKELLDMLAR AEREKK

Show sample condition

Sample

Temperature 303 (±1) K, pH 4.06 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Sac7d	[U-98% 15N]		1.18 mM
2	KCl			0.3 M
3	H2O			90 %
4	D2O			10 %
5	DSS			100 uM

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Calculated from 1 models in PDB: 1SAP, Strand ID: A Detail

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Release date

2003-10-05

Citation 1

Thermodynamics of Hydrophobic Core Packing in the Thermophile Proteins Sac7d and Sso7d
Clark, A.T., McCrary, B.S., Edmondson, S.P., Shriver, J.W.
J. Mol. Biol.

Show more 16 citaions

Citation 2

Binding of proteins to the minor groove of DNA: what are the structural and energetic determinants for kinking a basepair step?
Bosch, D., Campillo, M., Pardo, L.
J. Comput. Chem. (2003), 24, 682-691, PubMed 12666159 , DOI 10.1002/jcc.10200 , Abstract

Citation 3

DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricus
Napoli, A., Zivanovic, Y., Bocs, C., Buhler, C., Rossi, M., Forterre, P., Ciaramella, M.
Nucleic Acids Res. (2002), 30, 2656-2662, PubMed 12060682 , Abstract

Citation 4

DNA binding proteins Sac7d and Sso7d from Sulfolobus
Edmondson, S.P., Shriver, J.W.
Methods. Enzymol. (2001), 334, 129-145, PubMed 11398456 , DOI: , Abstract

Citation 5

The acid-induced folded state of Sac7d is the native state
Bedell, J.L., McCrary, B.S., Edmondson, S.P., Shriver, J.W.
Protein Sci. (2000), 9, 1878-1888, PubMed 11106160 , DOI 10.1110/ps.9.10.1878 , Abstract

Citation 6

Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs
Su, S., Gao, Y.G., Robinson, H., Liaw, Y.C., Edmondson, S.P., Shriver, J.W., Wang, A.H.
J. Mol. Biol. (2000), 303, 395-403, PubMed 11031116 , DOI 10.1006/jmbi.2000.4112 , Abstract

Citation 7

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 8

Effect of artificial periodicity in simulations of biomolecules under Ewald boundary conditions: a continuum electrostatics study
Hunenberger, P.H., McCammon, J.A.
Biophys. Chem. (1999), 78, 69-88, PubMed 10343384 , DOI: , Abstract

Citation 9

Molecular dynamics simulations of the hyperthermophilic protein sac7d from Sulfolobus acidocaldarius: contribution of salt bridges to thermostability
de Bakker, P.I., Hunenberger, P.H., McCammon, J.A.
J. Mol. Biol. (1999), 285, 1811-1830, PubMed 9917414 , DOI 10.1006/jmbi.1998.2397 , Abstract

Citation 10

The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA
Gao, Y.G., Su, S.Y., Robinson, H., Padmanabhan, S., Lim, L., McCrary, B.S., Edmondson, S.P., Shriver, J.W., Wang, A.H.
Nat. Struct. Biol. (1998), 5, 782-786, PubMed 9731772 , DOI 10.1038/1822 , Abstract

Citation 11

The hyperthermophile chromosomal protein Sac7d sharply kinks DNA
Robinson, H., Gao, Y.G., McCrary, B.S., Edmondson, S.P., Shriver, J.W., Wang, A.H.
Nature (1998), 392, 202-205, PubMed 9515968 , DOI 10.1038/32455 , Abstract

Citation 12

Linkage of protonation and anion binding to the folding of Sac7d
McCrary, B.S., Bedell, J., Edmondson, S.P., Shriver, J.W.
J. Mol. Biol. (1998), 276, 203-224, PubMed 9514720 , DOI 10.1006/jmbi.1998.1500 , Abstract

Citation 13

Overproduction of Sac7d and Sac7e reveals only Sac7e to be a DNA-binding protein with ribonuclease activity from the extremophilic archaeon Sulfolobus acidocaldarius
Kulms, D., Schafer, G., Hahn, U.
Biol. Chem. (1997), 378, 545-551, PubMed 9224936 , DOI: , Abstract

Citation 14

Hyperthermophile protein folding thermodynamics: differential scanning calorimetry and chemical denaturation of Sac7d
McCrary, B.S., Edmondson, S.P., Shriver, J.W.
J. Mol. Biol. (1996), 264, 784-805, PubMed 8980686 , DOI 10.1006/jmbi.1996.0677 , Abstract

Citation 15

Equilibrium DNA binding of Sac7d protein from the hyperthermophile Sulfolobus acidocaldarius: fluorescence and circular dichroism studies
McAfee, J.G., Edmondson, S.P., Zegar, I., Shriver, J.W.
Biochemistry (1996), 35, 4034-4045, PubMed 8672437 , DOI 10.1021/bi952555q , Abstract

Citation 16

Solution structure of the DNA-binding protein Sac7d from the hyperthermophile Sulfolobus acidocaldarius
Edmondson, S.P., Qiu, L., Shriver, J.W.
Biochemistry (1995), 34, 13289-13304, PubMed 7577913 , DOI: , Abstract

Citation 17

Gene cloning, expression, and characterization of the Sac7 proteins from the hyperthermophile Sulfolobus acidocaldarius
McAfee, J.G., Edmondson, S.P., Datta, P.K., Shriver, J.W., Gupta, R.
Biochemistry (1995), 34, 10063-10077, PubMed 7632679 , DOI: , Abstract

Hide non-primary 16 citaions

Related entities 1. Sac7d V30I monomer, : 1 : 8 : 74 entities Detail

Experiments performed 3 experiments Detail