BMRB: 5914

NMR structure of Methionine-Enkephalin in fast tumbling Bicelles/DMPG

Authors

Marcotte, I., Separovic, F., Auger, M., Gagne, S.M.

Assembly

Met-enkephalin

Entity

1. Met-enkephalin (polymer, Thiol state: not present), 5 monomers, 573.6619 Da Detail

YGGFM

Formula weight

573.6619 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.3 %, Completeness (bb): 91.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	83.3 % (25 of 30)	83.3 % (25 of 30)
Backbone	91.7 % (11 of 12)	91.7 % (11 of 12)
Sidechain	77.8 % (14 of 18)	77.8 % (14 of 18)
Aromatic	55.6 % (5 of 9)	55.6 % (5 of 9)

1. Met-enkephalin

YGGFM

Show sample condition

Sample

Pressure 1 atm, Temperature 295 K, pH 4.8

#	Name	Isotope labeling	Type	Concentration
1	Met-enkephalin			5.1 uM
2	(DMPC+DMPG)/DHPC 0.5:1 DMPC/DMPG 9:1 Lipid/peptide 25:1			10 % w/v
3	H2O			90 %
4	D2O			10 %

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Calculated from 80 models in PDB: 1PLX, Strand ID: A Detail

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Release date

2004-03-06

Citation

A multidimensional 1H NMR investigation of the conformation of methionine-enkephalin in fast-tumbling bicelles
Marcotte, I., Separovic, F., Auger, M., Gagne, S.M.
Biophys. J. (2004), 86, 1587-1600, PubMed 14990485 , DOI 10.1016/S0006-3495(04)74226-5 , Abstract

Experiments performed 2 experiments Detail