BMRBj - BMREntryMaster

Found 1 Document (1.309 seconds)

BMRB: 5930

1ASX

Backbone 1H, 15N, 13C Resonance Assignment of a-ADT

Authors

John, M., Heller, M., Coles, M., Bosch, G., Baumeister, W., Kessler, H.

Assembly

aADT

Entity

1. aADT (polymer, Thiol state: all free), 159 monomers, 17788.34 Da Detail

MSGIVIDKEK VHSKMPDVVK NAKIALIDSA LEIKKTEIEA KVQISDPSKI QDFLNQETNT FKQMVEKIKK SGANVVLCQK GIDDVAQHYL AKEGIYAVRR VKKSDMEKLA KATGAKIVTD LDDLTPSVLG EAETVEERKI GDDRMTFVMG CKNHHHHHH

Formula weight

17788.34 Da

Source organism

Thermoplasma acidophilum

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.7 %, Completeness: 41.7 %, Completeness (bb): 79.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	41.7 % (777 of 1862)	32.4 % (315 of 973)	43.9 % (317 of 722)	86.8 % (145 of 167)
Backbone	79.4 % (753 of 948)	92.6 % (299 of 323)	65.9 % (309 of 469)	92.9 % (145 of 156)
Sidechain	4.1 % (44 of 1065)	2.6 % (17 of 650)	6.7 % (27 of 404)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 78)	0.0 % (0 of 39)	0.0 % (0 of 39)
Methyl	4.3 % (8 of 188)	4.3 % (4 of 94)	4.3 % (4 of 94)

1. apical domain of the Thermosome

MSGIVIDKEK VHSKMPDVVK NAKIALIDSA LEIKKTEIEA KVQISDPSKI QDFLNQETNT FKQMVEKIKK SGANVVLCQK GIDDVAQHYL AKEGIYAVRR VKKSDMEKLA KATGAKIVTD LDDLTPSVLG EAETVEERKI GDDRMTFVMG CKNHHHHHH

Show sample condition

Sample #1

Temperature 315 (±0.2) K, pH 5.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	apical domain of the Thermosome	[U-15N]	0.7 ~ 1.0 mM
2	Na Phosphate		25 mM
3	NaCl		100 mM

Sample #2

Temperature 315 (±0.2) K, pH 5.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	apical domain of the Thermosome	[U-13C; U-15N]	0.7 ~ 1.0 mM
5	Na Phosphate		25 mM
6	NaCl		100 mM

Release date

2004-04-06

Citation 1

Backbone 1H, 15N and 13C resonance assignments of alpha-ADT and beta-ADT
Kessler, H., John, M., Heller, M., Coles, M., Bosch, G., Baumeister, W.
J. Biomol. NMR (2004), 29, 209-210, PubMed 15014236 , DOI 10.1023/B:JNMR.0000019245.41652.9f , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. aADT, : 1 : 1 : 292 entities Detail

Interaction partners 1. aADT, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5930_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5930_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	aADT_bb	Warning	734		93.7 (chain: 1, length: 159)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: aADT_bb
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 159, Sequence coverage: 93.7%
- Total number of assignments
  - ¹³C chemical shifts: 290
  - ¹H chemical shifts: 299
  - ¹⁵N chemical shifts: 145
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (1.309 seconds)

BMRB: 5930

Sample #1

Sample #2

Related entities 1. aADT, : 1 : 1 : 292 entities Detail

Interaction partners 1. aADT, : 1 interactors Detail

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	722	290	40.2
¹H chemical shifts	973	299	30.7
¹⁵N chemical shifts	171	145	84.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	318	290	91.2
¹H chemical shifts	323	299	92.6
¹⁵N chemical shifts	156	145	92.9