BMRBj - BMREntryMaster

Found 1 Document (1.243 seconds)

BMRB: 5936

1E0R

Backbone 1H, 15N, 13C Resonance Assignment of b-ADT

Authors

John, M., Heller, M., Coles, M., Bosch, G., Baumeister, W., Kessler, H.

Assembly

bADT

Entity

1. bADT (polymer, Thiol state: not present), 159 monomers, 17706.11 Da Detail

MSGIIVDKEK VHPGMPDVVK DAKIALLDAP LEIKKPEFDT NLRIEDPSMI QKFLAQEENM LREMVDKIKS VGANVVITQK GIDDMAQHYL SRAGIYAVRR VKKSDMDKLA KATGASIVST IDEISSSDLG TAERVEQVKV GEDYMTFVTG SKNHHHHHH

Formula weight

17706.11 Da

Source organism

Thermoplasma acidophilum

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.8 %, Completeness: 38.6 %, Completeness (bb): 68.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	38.6 % (708 of 1833)	31.0 % (296 of 954)	40.2 % (288 of 716)	76.1 % (124 of 163)
Backbone	68.6 % (648 of 944)	78.0 % (251 of 322)	58.5 % (274 of 468)	79.9 % (123 of 154)
Sidechain	7.4 % (77 of 1039)	7.3 % (46 of 632)	7.5 % (30 of 398)	11.1 % (1 of 9)
Aromatic	3.5 % (3 of 86)	7.0 % (3 of 43)	0.0 % (0 of 43)
Methyl	5.4 % (10 of 186)	4.3 % (4 of 93)	6.5 % (6 of 93)

1. apical domain of the Thermosome

MSGIIVDKEK VHPGMPDVVK DAKIALLDAP LEIKKPEFDT NLRIEDPSMI QKFLAQEENM LREMVDKIKS VGANVVITQK GIDDMAQHYL SRAGIYAVRR VKKSDMDKLA KATGASIVST IDEISSSDLG TAERVEQVKV GEDYMTFVTG SKNHHHHHH

Show sample condition

Sample #1

Temperature 315 (±0.2) K, pH 7.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	apical domain of the Thermosome	[U-15N]	0.7 ~ 1.0 mM
2	Na Phosphate		25 mM
3	NaCl		100 mM

Sample #2

Temperature 315 (±0.2) K, pH 7.8 (±0.2)

#	Name	Isotope labeling	Concentration
4	apical domain of the Thermosome	[U-13C; U-15N]	0.7 ~ 1.0 mM
5	Na Phosphate		25 mM
6	NaCl		100 mM

Release date

2004-04-06

Citation 1

Backbone 1H, 15N and 13C resonance assignments of alpha-ADT and beta-ADT
John, M., Heller, M., Coles, M., Bosch, G., Baumeister, W., Kessler, H.
J. Biomol. NMR (2004), 29, 209-210, PubMed 15014236 , DOI 10.1023/B:JNMR.0000019245.41652.9f , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI 10.1007/bf00211777 , Abstract

Hide non-primary 1 citaion

Related entities 1. bADT, : 1 : 295 entities Detail

Interaction partners 1. bADT, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5936_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5936_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	bADT_bb	Warning	605		83.6 (chain: 1, length: 159)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: bADT_bb
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 159, Sequence coverage: 83.6%
- Total number of assignments
  - ¹³C chemical shifts: 248
  - ¹H chemical shifts: 241
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	101	0	0.0
¹H chemical shifts	101	0	0.0

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Found 1 Document (1.243 seconds)

BMRB: 5936

Sample #1

Sample #2

Related entities 1. bADT, : 1 : 295 entities Detail

Interaction partners 1. bADT, : 1 interactors Detail

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	716	248	34.6
¹H chemical shifts	954	241	25.3
¹⁵N chemical shifts	169	116	68.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	318	248	78.0
¹H chemical shifts	322	241	74.8
¹⁵N chemical shifts	154	116	75.3