BMRB: 5962

Chemical shifts assignments of domain 5 of the ai5gamma group II intron

Authors

Sigel, R.K. O., Sashital, D.G., Abramowitz, D.L., Palmer, A.G., Butcher, S.E., Pyle, A.

Assembly

wildtype domain 5 of the ai5gamma group II intron

Entity

1. wildtype domain 5 of the ai5gamma group II intron (polymer, Thiol state: not present), 36 monomers, 11683.93 Da Detail

GGAGCCGUAU GCGAUGAAAG UCGCACGUAC GGUUCC

Formula weight

11683.93 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 64.9 %, Completeness (bb): 51.8 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	64.9 % (371 of 572)	82.1 % (257 of 313)	40.4 % (97 of 240)	89.5 % (17 of 19)
Suger, PO4	51.8 % (205 of 396)	77.8 % (168 of 216)	20.6 % (37 of 180)
Nucleobase	94.3 % (166 of 176)	91.8 % (89 of 97)	100.0 % (60 of 60)	89.5 % (17 of 19)
Aromatic	96.2 % (152 of 158)	94.9 % (75 of 79)	100.0 % (60 of 60)	89.5 % (17 of 19)

1. Domain 5

GGAGCCGUAU GCGAUGAAAG UCGCACGUAC GGUUCC

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Domain 5	[U-13C; U-15N]		0.4 ~ 0.7 mM
2	KCl			100 mM
3	Edta			10 mM

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Release date

2004-03-06

Citation

Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif
Sigel, R.K. O., Sashital, D.G., Abramowitz, D.L., Palmer, A.G., Butcher, S.E., Pyle, A.
Nat. Struct. Mol. Biol. (2004), 11, 187-192, PubMed 14745440 , DOI 10.1038/nsmb717 , Abstract

Related entities 1. wildtype domain 5 of the ai5gamma group II intron, : 1 : 5 : 1 entities Detail

Experiments performed 7 experiments Detail