BMRBj - BMREntryMaster

Found 1 Document (1.088 seconds)

BMRB: 5974

2JOL

Assignment of the 1H, 13C and 15N resonances of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei

Authors

Wu, H., Williams, C., Upadhyay, A., Galyov, E.E., Stefan, B.

Assembly

BopE

Entity

1. BopE (polymer, Thiol state: all free), 187 monomers, 20182.19 Da Detail

GSTGDAKQAI RHFVDEAVKQ VAHARTPEIR QDAEFGRQVY EATLCAIFSE AKDRFCMDPA TRAGNVRPAF IEALGDAARA TGLPGADKQG VFTPSGAGTN PLYTEIRLRA DTLMGAELAA RPEYRELQPY ARQQAIDLVA NALPAERSNT LVEFRQTVQT LEATYRRAAQ DASRDEKGAT NAADGAA

Formula weight

20182.19 Da

Source organism

Burkholderia pseudomallei

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 78.3 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.3 % (1599 of 2041)	71.4 % (754 of 1056)	84.8 % (671 of 791)	89.7 % (174 of 194)
Backbone	97.1 % (1072 of 1104)	97.6 % (369 of 378)	96.5 % (529 of 548)	97.8 % (174 of 178)
Sidechain	62.8 % (698 of 1111)	56.8 % (385 of 678)	75.1 % (313 of 417)	0.0 % (0 of 16)
Aromatic	3.4 % (4 of 118)	3.4 % (2 of 59)	3.4 % (2 of 59)
Methyl	82.2 % (171 of 208)	83.7 % (87 of 104)	80.8 % (84 of 104)

1. BopE

Show sample condition

Sample

Temperature 298 (±0.3) K, pH 5.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	BopE	[U-95% 13C; U-90% 15N]		1.0 mM

Release date

2004-04-06

Citation

Assignment of the 1H, 13C and 15N resonances of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei
Wu, H., Williams, C., Upadhyay, A., Galyov, E.E., Bagby, S.
J. Biomol. NMR (2004), 29, 215-216, PubMed 15014239 , DOI 10.1023/B:JNMR.0000019244.41446.14 , Abstract

Related entities 1. BopE, : 1 : 3 : 1 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5974_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5974_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1757		98.4 (chain: 1, length: 187)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 187, Sequence coverage: 98.4%
- Total number of assignments
  - ¹⁵N chemical shifts: 175
  - ¹H chemical shifts: 910
  - ¹³C chemical shifts: 672
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	212	175	82.5
¹H chemical shifts	1056	738	69.9
¹³C chemical shifts	791	672	85.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	178	175	98.3
¹H chemical shifts	378	366	96.8
¹³C chemical shifts	374	359	96.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	34	0	0.0
¹H chemical shifts	678	372	54.9
¹³C chemical shifts	417	313	75.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	106	86	81.1
¹³C chemical shifts	106	84	79.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	0	0.0
¹³C chemical shifts	59	0	0.0

Keywords guanine nucleotide exchange factor, Burkholderia pseudomallei, type III secretion, Rho GTPase, melioidosis

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Found 1 Document (1.088 seconds)

BMRB: 5974

Sample

Related entities 1. BopE, : 1 : 3 : 1 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail