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BMRB: 6003

1ZWT

Complete Resonance Assignments of Bundlin (BfpA) from the Bundle- forming Pilus of Enteropathogenic Escherichia coli

Authors

Ramboarina, S., Fernandes, P., Simpson, P.J., Frankel, G., Donnenberg, M.S., Matthews, S.J.

Assembly

Bundlin forming pilus subunit

Entity

1. Bundlin forming pilus subunit (polymer, Thiol state: all disulfide bound), 164 monomers, 17297.82 Da Detail

HHHHHHMEQS ASDSNKSQNA ISEVMSATSA INGLYIGQTS YSGLDSTILL NTSAIPDNYK DTTNKKITNP FGGELNVGPA NNNTAFGYYL TLTRLDKAAC VSLATLNLGT SAKGYGVNIS GENNITSFGN SADQAAKSTA ITPAEAATAC KNTDSTNKVT YFMK

Formula weight

17297.82 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS100:SG	1:CYS150:SG

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 85.9 %, Completeness (bb): 91.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.9 % (1515 of 1764)	84.9 % (762 of 898)	88.6 % (606 of 684)	80.8 % (147 of 182)
Backbone	91.7 % (895 of 976)	91.7 % (308 of 336)	91.9 % (441 of 480)	91.3 % (146 of 160)
Sidechain	80.9 % (760 of 940)	80.8 % (454 of 562)	85.7 % (305 of 356)	4.5 % (1 of 22)
Aromatic	75.0 % (90 of 120)	75.0 % (45 of 60)	75.0 % (45 of 60)
Methyl	94.9 % (167 of 176)	96.6 % (85 of 88)	93.2 % (82 of 88)

1. bundlin

HHHHHHMEQS ASDSNKSQNA ISEVMSATSA INGLYIGQTS YSGLDSTILL NTSAIPDNYK DTTNKKITNP FGGELNVGPA NNNTAFGYYL TLTRLDKAAC VSLATLNLGT SAKGYGVNIS GENNITSFGN SADQAAKSTA ITPAEAATAC KNTDSTNKVT YFMK

Show sample condition

Sample

Temperature 303 (±2) K, pH 5.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	bundlin	[U-95% 13C; U-95% 15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: -0.49 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: -0.49 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.11 ppm, Outliers: 3 Detail

Release date

2004-07-05

Citation

Complete resonance assignments of bundlin (BfpA) from the bundle-forming pilus of enteropathogenic Escherichia coli
Ramboarina, S., Fernandes, P., Simpson, P.J., Frankel, G., Donnenberg, M.S., Matthews, S.J.
J. Biomol. NMR (2004), 29, 427-428, PubMed 15213452 , DOI 10.1023/B:JNMR.0000032511.89525.64 , Abstract

Related entities 1. Bundlin forming pilus subunit, : 1 : 2 entities Detail

Experiments performed 10 experiments Detail

1 1H-15N HSQC