1H, 13C and 15N resonance assignments of the methylated N-terminal 16kDa domain of Escherichia coli Ada Protein
MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFXRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF KATTGMTPKA WQQAWRARRL RESLAK
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:SMC38:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS42:SG | 2:ZN1:ZN |
3 | na | sing | 1:CYS69:SG | 2:ZN1:ZN |
4 | na | sing | 1:CYS72:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.1 % (1581 of 1699) | 92.8 % (826 of 890) | 93.3 % (611 of 655) | 93.5 % (144 of 154) |
Backbone | 96.1 % (823 of 856) | 96.2 % (276 of 287) | 96.5 % (416 of 431) | 94.9 % (131 of 138) |
Sidechain | 91.2 % (897 of 984) | 91.2 % (550 of 603) | 91.5 % (334 of 365) | 81.3 % (13 of 16) |
Aromatic | 79.1 % (106 of 134) | 88.1 % (59 of 67) | 70.3 % (45 of 64) | 66.7 % (2 of 3) |
Methyl | 98.6 % (146 of 148) | 98.6 % (73 of 74) | 98.6 % (73 of 74) |
1. Escherichia coli Ada Protein
MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFXRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF KATTGMTPKA WQQAWRARRL RESLAKTemperature 303 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Escherichia coli Ada Protein | [U-98% 13C; U-98% 15N] | 1.0 mM |