BMRB: 6054

1H, 13C and 15N resonance assignments of the methylated N-terminal 16kDa domain of Escherichia coli Ada Protein

Authors

Takinowaki, H., Matsuda, Y., Yoshida, T., Kobayashi, Y., Ohkubo, T.

Assembly

the methylated N-terminal 16kDa domain of Ada Protein

Entity

1. Escherichia coli Ada Protein (polymer, Thiol state: free and other bound), 146 monomers, 16636.92 Da Detail

MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFXRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF KATTGMTPKA WQQAWRARRL RESLAK

2. ZINC ION (non-polymer), 65.409 Da

Total weight

16702.328 Da

Max. entity weight

16636.92 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:SMC38:SG	2:ZN1:ZN
2	na	sing	1:CYS42:SG	2:ZN1:ZN
3	na	sing	1:CYS69:SG	2:ZN1:ZN
4	na	sing	1:CYS72:SG	2:ZN1:ZN

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 93.1 %, Completeness (bb): 96.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.1 % (1581 of 1699)	92.8 % (826 of 890)	93.3 % (611 of 655)	93.5 % (144 of 154)
Backbone	96.1 % (823 of 856)	96.2 % (276 of 287)	96.5 % (416 of 431)	94.9 % (131 of 138)
Sidechain	91.2 % (897 of 984)	91.2 % (550 of 603)	91.5 % (334 of 365)	81.3 % (13 of 16)
Aromatic	79.1 % (106 of 134)	88.1 % (59 of 67)	70.3 % (45 of 64)	66.7 % (2 of 3)
Methyl	98.6 % (146 of 148)	98.6 % (73 of 74)	98.6 % (73 of 74)

1. Escherichia coli Ada Protein

MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFXRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF KATTGMTPKA WQQAWRARRL RESLAK

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Escherichia coli Ada Protein	[U-98% 13C; U-98% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.31 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.31 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2003-12-17

Citation

1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein
Takinowaki, H., Matsuda, Y., Yoshida, T., Kobayashi, Y., Ohkubo, T.
J. Biomol. NMR (2004), 29, 447-448, PubMed 15213462 , DOI 10.1023/B:JNMR.0000032549.04619.87 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Escherichia coli Ada Protein, : 1 : 2 : 8 entities Detail

Interaction partners 1. Escherichia coli Ada Protein, : 8 interactors Detail

Experiments performed 6 experiments Detail