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BMRB: 6086

1H, 13C and 15N assignments of the tandem WW domains of human MAGI-1/BAP-1

Authors

Kato, Y.K., Akai, A., Suzuki, R., Hosokawa, H., Ninomiya, H., Masaki, T., Nagata, K., Tanokura, M.

Assembly

recombinant MAGI-1 tandem WW domain region

Entity

1. recombinant MAGI-1 tandem WW domain region (polymer, Thiol state: not present), 101 monomers, 11799.65 Da Detail

MAEDNLGPLP ENWEMAYTEN GEVYFIDHNT KTTSWLDPRS LNKQQKPLEE SEDDEGVHTE ELDSELELPA GWEKIEDPVY GIYYVDHINR KTQYENPVLE A

Formula weight

11799.65 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.8 %, Completeness (bb): 95.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.8 % (1006 of 1186)	82.2 % (509 of 619)	86.5 % (398 of 460)	92.5 % (99 of 107)
Backbone	95.9 % (568 of 592)	94.0 % (188 of 200)	96.6 % (288 of 298)	97.9 % (92 of 94)
Sidechain	77.1 % (532 of 690)	76.6 % (321 of 419)	79.1 % (204 of 258)	53.8 % (7 of 13)
Aromatic	59.4 % (63 of 106)	77.4 % (41 of 53)	38.0 % (19 of 50)	100.0 % (3 of 3)
Methyl	88.0 % (81 of 92)	91.3 % (42 of 46)	84.8 % (39 of 46)

1. membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1

MAEDNLGPLP ENWEMAYTEN GEVYFIDHNT KTTSWLDPRS LNKQQKPLEE SEDDEGVHTE ELDSELELPA GWEKIEDPVY GIYYVDHINR KTQYENPVLE A

Show sample condition

Sample

Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

LACS Plot; CA

Referencing offset: -0.52 ppm, Outliers: 3 Detail

LACS Plot; CB

Referencing offset: -0.52 ppm, Outliers: 3 Detail

LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Release date

2004-07-28

Citation

1H, 13C and 15N assignments of the tandem WW domains of human MAGI-1/BAP-1
Kato, Y., Akai, A., Suzuki, R., Hosokawa, H., Ninomiya, H., Masaki, T., Nagata, K., Tanokura, M.
J. Biomol. NMR (2004), 29, 539-540, PubMed 15243188 , DOI 10.1023/B:JNMR.0000034336.24315.0f , Abstract

Related entities 1. recombinant MAGI-1 tandem WW domain region, : 2 : 237 entities Detail

Interaction partners 1. recombinant MAGI-1 tandem WW domain region, : 17 interactors Detail

More details for 17 interactors identified by 13 citations

Experiments performed 11 experiments Detail

1 1H-15N HSQC

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

2 HN(CO)CA

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

3 HNCA

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

4 CBCA(CO)NH

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

5 HNCACB

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

6 HNCO

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

7 (HCA)CO(CA)NH

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

8 C(CO)NH

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

9 H(CCO)NH

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

10 HCCH-TOCSY

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

11 HCCH-COSY

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Temperature 310 (±0.3) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains 1			2.5 ~ 3.0 mM
2	sodium phosphaste			20 mM
3	NaCl			100 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6086_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6086_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift1	Warning	1085		99.0 (chain: 1, length: 101)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 101, Sequence coverage: 99.0%
- Total number of assignments
  - ¹H chemical shifts: 592
  - ¹³C chemical shifts: 395
  - ¹⁵N chemical shifts: 98
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords MAGI-1, WW domain