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BMRB: 6092


6092
1GM1
1H, 13C, and 15N Chemical Shift Assignments for a complex of PDZ2 from PTP-BL with the C-terminus of RIL (reversion induced LIM)
Authors
Walma, T., Vuister, G.
Assembly
PDZ2 from PTP-BL
Entity
1. Second PDZ domain from PTP-BL (polymer, Thiol state: not present), 102 monomers, 10837.16 Da Detail

MHHHHHHMKP GDTFEVELAK TDGSLGISVT GGVNTSVRHG GIYVKAIIPK GAAESDGRIH KGDRVLAVNG VSLEGATHKQ AVETLRNTGQ VVHLLLEKGQ VP


2. C-terminus from RIL (polymer, Thiol state: not present), 12 monomers, 1301.528 Da Detail

VAVYPNAKVE LV


Total weight
12138.688 Da
Max. entity weight
10837.16 Da
Source organism
Mus musculus
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS
Data set
assigned_chemical_shifts, heteronucl_T1_relaxation, heteronucl_T2_relaxation, heteronucl_NOEs
Chem. Shift Complete
Sequence coverage: 83.3 %, Completeness: 70.5 %, Completeness (bb): 68.6 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All70.5 % (886 of 1257)77.6 % (500 of 644)58.5 % (290 of 496)82.1 % (96 of 117)
Backbone68.6 % (464 of 676)82.8 % (197 of 238)54.0 % (177 of 328)81.8 % (90 of 110)
Sidechain73.1 % (498 of 681)74.6 % (303 of 406)70.5 % (189 of 268)85.7 % (6 of 7)
Aromatic36.4 % (24 of 66)48.5 % (16 of 33)24.2 % (8 of 33)
Methyl80.3 % (122 of 152)81.6 % (62 of 76)78.9 % (60 of 76)

1. Second PDZ domain from PTP-BL

MHHHHHHMKP GDTFEVELAK TDGSLGISVT GGVNTSVRHG GIYVKAIIPK GAAESDGRIH KGDRVLAVNG VSLEGATHKQ AVETLRNTGQ VVHLLLEKGQ VP

2. C-terminus from RIL

VAVYPNAKVE LV

Show sample condition
Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.2)


#NameIsotope labelingTypeConcentration
1Second PDZ domain from PTP-BL[U-15N; U-13C]1 mM
2C-terminus from RIL3.55 mM
3K2HPO4/KH2PO450 mM
4KCl50 mM

LACS Plot; CA
Referencing offset: -0.03 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.03 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.05 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 35 models in PDB: 1GM1, Strand ID: A Detail

Heteronucl. T1
82 T1 values in 1 lists
Coherence Sz, Field strength (1H) 600 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.2) Detail
Heteronucl. T2
82 T2 values in 1 lists
Coherence na, Field strength (1H) 600 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.2) Detail
Heteronucl. NOE
82 NOE values in 1 lists
Value type na, Field strength (1H) 600 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.2) Detail
Heteronucl. T1/T2
81 T1/T2 values in 1 lists
Field strength (1H) 600 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.2) Detail
Release date
2011-08-10
Citation
A closed binding pocket and global destabilization modify the binding properties of an alternatively spliced form of the second PDZ domain of PTP-BL
Walma, T., Aelen, J., Nabuurs, S.B., Oostendorp, M., van den Berk, L., Hendriks, W., Vuister, G.W.
Structure (2004), 12, 11-20, PubMed 14725761 , DOI: ,
Related entities 1. Second PDZ domain from PTP-BL, : 2 : 334 entities Detail
More details for 336 related entities
Related entities 2. C-terminus from RIL, : 8 : 1 entities Detail
More details for 9 related entities
Interaction partners 1. Second PDZ domain from PTP-BL, : 8 : 1 interactors Detail
More details for 9 interactors identified by 6 citations
Interaction partners 2. C-terminus from RIL, : 1 interactors Detail
More details for 1 interactors identified by 1 citations
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