BMRBj - BMREntryMaster

Found 1 Document (1.065 seconds)

BMRB: 6099

Backbone 1H, 13C, and 15N chemical shift assignments for the activation domain of the transcriptional activator VP16

Authors

Jonker, H., Folkers, G., Wechselberger, R., Boelens, R., Kaptein, R.

Assembly

VP16ad

Entity

1. VP16ad (polymer, Thiol state: not present), 80 monomers, 8363.751 Da Detail

STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG DSPGPGFTPH DSAPYGALDT ADFEFEQMFT DALGIDEYGG

Formula weight

8363.751 Da

Source organism

Human alphaherpesvirus 1

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 71.6 %, Completeness (bb): 95.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.6 % (583 of 814)	69.0 % (283 of 410)	69.6 % (229 of 329)	94.7 % (71 of 75)
Backbone	95.9 % (449 of 468)	96.3 % (158 of 164)	95.7 % (220 of 230)	95.9 % (71 of 74)
Sidechain	48.3 % (201 of 416)	50.8 % (125 of 246)	45.0 % (76 of 169)	0.0 % (0 of 1)
Aromatic	0.0 % (0 of 78)	0.0 % (0 of 39)	0.0 % (0 of 39)
Methyl	41.7 % (30 of 72)	41.7 % (15 of 36)	41.7 % (15 of 36)

1. herpes simplex virion protein 16

STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG DSPGPGFTPH DSAPYGALDT ADFEFEQMFT DALGIDEYGG

Show sample condition

Sample

Temperature 298 (±1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	herpes simplex virion protein 16	[U-13C; U-15N]	0.5 ~ 1.0 mM
2	KCl		50 mM
3	KHxPO4		50 mM
4	D5-Glycin		2 M
5	Protease Inhibitor cocktail		0.0 ~ 0.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 2 Detail

Release date

2004-09-27

Citation

Structural and functional studies on transcriptional activation - Interactions of the cofactor PC4 and activator VP16
Jonker, H.

Related entities 1. VP16ad, : 2 : 1 entities Detail

Interaction partners 1. VP16ad, : 9 interactors Detail

More details for 9 interactors identified by 4 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6099_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6099_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_VP16_298K_LS	OK	591		97.5 (chain: 1, length: 80)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: chemical_shift_VP16_298K_LS
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 80, Sequence coverage: 97.5%
- Total number of assignments
  - ¹H chemical shifts: 294
  - ¹⁵N chemical shifts: 73
  - ¹³C chemical shifts: 224
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	410	276	67.3
¹⁵N chemical shifts	75	73	97.3
¹³C chemical shifts	329	224	68.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	164	161	98.2
¹⁵N chemical shifts	74	73	98.6
¹³C chemical shifts	160	156	97.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	246	115	46.7
¹⁵N chemical shifts	1	0	0.0
¹³C chemical shifts	169	68	40.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	9	23.1
¹³C chemical shifts	39	9	23.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	0	0.0
¹³C chemical shifts	39	0	0.0

Keywords PC4 cofactor VP16 activator transcription

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Found 1 Document (1.065 seconds)

BMRB: 6099

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. VP16ad, : 2 : 1 entities Detail

Interaction partners 1. VP16ad, : 9 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail